Detail Information for IndEnz0002008812
IED ID IndEnz0002008812
Enzyme Type ID protease008812
Protein Name Extracellular small neutral protease
EC 3.4.24.77
Extracellular metalloprotease
Snapalysin
Gene Name snpA lmp mprA mprA2 prt
Organism Streptomyces lividans
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces lividans
Enzyme Sequence MRITLPLLSTAVGLGLTAAVLGTGPAATAAAPQEPVRAAQLGYQPSAGSGEDAAANRAFFEAVVKSVAEKRAANPSAAAAVTVYYSATNAPSFRSQISRSAQIWNSSVSNVRLAESSSGADFAYYEGNDSRGSYASTDGHGSGYIFLDYRQNQQYDSTRVTAHETGHVLGLPDHYSGPCSELMSGGGPGPSCTNPYPNSTERSRVNQLWAYGFQAALDKALEKASQR
Enzyme Length 227
Uniprot Accession Number P0A3Z8
Absorption
Active Site ACT_SITE 164; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.; EC=3.4.24.77;
DNA Binding
EC Number 3.4.24.77
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (1); Metal binding (5); Propeptide (1); Sequence conflict (1); Signal peptide (1)
Keywords Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification PTM: The N-terminus is blocked.
Signal Peptide SIGNAL 1..29; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 23,765
Kinetics
Metal Binding METAL 156; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 158; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 163; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 173; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406
Rhea ID
Cross Reference Brenda