IED ID | IndEnz0002008812 |
Enzyme Type ID | protease008812 |
Protein Name |
Extracellular small neutral protease EC 3.4.24.77 Extracellular metalloprotease Snapalysin |
Gene Name | snpA lmp mprA mprA2 prt |
Organism | Streptomyces lividans |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces lividans |
Enzyme Sequence | MRITLPLLSTAVGLGLTAAVLGTGPAATAAAPQEPVRAAQLGYQPSAGSGEDAAANRAFFEAVVKSVAEKRAANPSAAAAVTVYYSATNAPSFRSQISRSAQIWNSSVSNVRLAESSSGADFAYYEGNDSRGSYASTDGHGSGYIFLDYRQNQQYDSTRVTAHETGHVLGLPDHYSGPCSELMSGGGPGPSCTNPYPNSTERSRVNQLWAYGFQAALDKALEKASQR |
Enzyme Length | 227 |
Uniprot Accession Number | P0A3Z8 |
Absorption | |
Active Site | ACT_SITE 164; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.; EC=3.4.24.77; |
DNA Binding | |
EC Number | 3.4.24.77 |
Enzyme Function | |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Chain (1); Disulfide bond (1); Metal binding (5); Propeptide (1); Sequence conflict (1); Signal peptide (1) |
Keywords | Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
Modified Residue | |
Post Translational Modification | PTM: The N-terminus is blocked. |
Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 23,765 |
Kinetics | |
Metal Binding | METAL 156; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 158; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 163; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 173; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406 |
Rhea ID | |
Cross Reference Brenda |