| IED ID | IndEnz0002008815 |
| Enzyme Type ID | protease008815 |
| Protein Name |
Serine protease inhibitor 28Dc Serpin 28Dc Serpin-28D |
| Gene Name | Spn28Dc Spn28D CG7219 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MWRLLLALLLVSSVCCESELFRDDLRTPETMAYINGLMQRRHQMQQEAQQHIQAIPPAVPLQSPGLVNGLGNQNDPALNRISGTSVKPSNLPAAYSNGYVDLATSDRIANSVLNFANILGQHLANGKTQIYSPLSIVHSLALLLLGAKGRSYEELSTVFDIPDTSRLHEQFGLMLQDLQQPTREAISAGRPLTDWRASSAMRSNRRAQRPGAHEVHLANGLFTQTGYTLNPDYRRVIVEVYASDLQIQDFEGSPATARYNINAYVAQHTKNHIENIIASDIPQTTRMILANALYFKAFWETDFIESATRPDNFYPNGEGTEPVMRVQMMATGGAYPYHEDHELGCKIIGLPYRGNLSTMYIIQPFKSSVRELMALQKRLTADKIESMISRMYRRAALVAFPKMHLTESVNLKTVMQRMGLGGIFSAVQNDLSLIATNEATRTNALGGNSLQNLEAQRRAGTGGARSDLVVDDIVHKVDFTVNEQGTEAAASSVTYLKKSGPDVLFRGDTPFMVLVRHDPTKLVLFYGLINEPPAAA |
| Enzyme Length | 536 |
| Uniprot Accession Number | Q9VLU4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Serine protease inhibitor which is required for pupal viability and plays an essential role in regulating the melanization reaction (PubMed:18801354, PubMed:22227521). Inhibits spontaneous melanization and appears to be involved in the melanization immune response to physical wounding in larvae and adults (PubMed:18801354, PubMed:22227521). Acts by negatively regulating the Hayan-phenoloxidase (PPO1) cascade in the hemolymph and possibly the trachea (PubMed:18801354, PubMed:22227521). May function by controlling the initial release of the activated form of PPO1, phenoloxidase (PO) and thus maintains PO availability for processes such as wound response and pigmentation (PubMed:18801354). {ECO:0000269|PubMed:18801354, ECO:0000269|PubMed:22227521}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (1); Signal peptide (1); Site (1) |
| Keywords | Developmental protein;Glycoprotein;Immunity;Melanin biosynthesis;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal |
| Interact With | |
| Induction | INDUCTION: Up-regulated by injury in larvae and adults. {ECO:0000269|PubMed:18801354}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:18801354}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11529271; 11606746; 12032070; 12364793; 14605208; 14667416; 16581772; 19109990; 19223926; 19849829; 20220848; 21074052; 21480662; 21571080; 21683256; 23071443; 23550122; 23840627; 23944235; 25312911; 26173873; 27172210; 29670218; 31722958; 32598400; 33914801; |
| Motif | |
| Gene Encoded By | |
| Mass | 59,350 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |