| IED ID | IndEnz0002008821 |
| Enzyme Type ID | protease008821 |
| Protein Name |
Seminase EC 3.4.21.- |
| Gene Name | Sems CG10586 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MKRLLFLFLLAGILINNHALQHNETIDLKKLAKIVLPPAYQTRVIGGRVTTNAKLGGYLVAMRYFNNFICGGTLIHELIVLTAAHCFEDRAEKEAWSVDGGISRLSEKGIRRQVKRFIKSAQFKMVTMNMDVAVVLLNRPMVGKNIGTLSLCSTALTPGQTMDVSGWGMTNPDDEGPGHMLRTVSVPVIEKRICREAYRESVSISDSMFCASVLGKKDACTYDSGGPLVYEKQVCGIVSFGIGCASRRYPGVYTDVHYVKPFIVKGIKALLSRSR |
| Enzyme Length | 275 |
| Uniprot Accession Number | Q9VP95 |
| Absorption | |
| Active Site | ACT_SITE 85; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 131; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 224; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Seminal fluid protease which is required for cleavage and probably also activation of the metalloprotease Semp1 (PubMed:22253601, PubMed:24514904). Also required for a number of female post-mating responses independent of Semp1 including egg laying and sperm usage (PubMed:22253601). {ECO:0000269|PubMed:22253601, ECO:0000269|PubMed:24514904}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Erroneous initiation (1); Glycosylation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22253601}. Note=Secreted into seminal fluid. {ECO:0000269|PubMed:22253601}. |
| Modified Residue | |
| Post Translational Modification | PTM: Undergoes cleavage in the male during mating with a cleaved product detected in the ejaculatory duct and/or bulb of males by 8-10 minutes after the start of mating. Further cleavage occurs in the mated female. {ECO:0000269|PubMed:22253601}. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12568721; 17996400; 18666829; 20220848; 21074052; 21940639; 23071443; 23087839; 23109270; 23555301; 23944235; 24453993; 24973093; 25740935; 27172210; 30217614; 30367934; 31991034; 32672537; 33081607; 33827210; |
| Motif | |
| Gene Encoded By | |
| Mass | 30,337 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |