| IED ID |
IndEnz0002008862 |
| Enzyme Type ID |
protease008862 |
| Protein Name |
Serine protease inhibitor Rv3364c
|
| Gene Name |
Rv3364c LH57_18375 |
| Organism |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Taxonomic Lineage |
cellular organisms
Bacteria
Terrabacteria group
Actinobacteria
Actinomycetia (high G+C Gram-positive bacteria)
Corynebacteriales
Mycobacteriaceae
Mycobacterium
Mycobacterium tuberculosis complex
Mycobacterium tuberculosis
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
|
| Enzyme Sequence |
MKARLPDSPLDWLVSKFAREVPGVAHALLVSVDGLPVAASEHLPRERADQLAAVTSGLASLAGGAAQLFDGGQVLQSVVEMQNGYLLLMQVGDGSALAALAATGCDIGQIGYEMAILVERVGGVVQSCRR |
| Enzyme Length |
130 |
| Uniprot Accession Number |
O50393 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
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| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
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| Enzyme Function |
FUNCTION: Effector protein that binds to the host serine protease cathepsin G on the macrophage cell membrane, inhibiting its enzymatic activity and the downstream activation of caspase-1-dependent apoptosis. Thus prevents macrophage pyroptosis and helps M.tuberculosis survival within host cells. {ECO:0000269|PubMed:22275911}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1) |
| Keywords |
Host cytoplasm;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Virulence |
| Interact With |
|
| Induction |
INDUCTION: Component of the signal transduction operon Rv3361c-Rv3365c that is highly up-regulated during M.tuberculosis infection of macrophages. {ECO:0000269|PubMed:22275911}. |
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22275911}. Host cytoplasm {ECO:0000269|PubMed:22275911}. Note=Is translocated into the cytoplasm of macrophages. {ECO:0000269|PubMed:22275911}. |
| Modified Residue |
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| Post Translational Modification |
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| Signal Peptide |
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| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
13,440 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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