| IED ID |
IndEnz0002008864 |
| Enzyme Type ID |
protease008864 |
| Protein Name |
Transcription factor STP2
|
| Gene Name |
STP2 YHR006W |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Saccharomycotina (true yeasts)
Saccharomycetes
Saccharomycetales
Saccharomycetaceae
Saccharomyces
Saccharomyces cerevisiae (Baker's yeast)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
|
| Enzyme Sequence |
MPILSLSSTRNSVLTRIYDYLKALVQQVIVPNVEDDKSSKSTPFEKLEPAKQNHPQKDCCATEKDDLVDVSELFPKQNNKQLSLTSKSSVVPCALNLDNLETPFSIKIDNNGAVTTQLNLDEPILRGPSRGEPAKLQNDLISSPPLEESYINNDQYKALFPSNFLPITPVSSVITPASKKSIDESPLSDEVQGIADESSETLPYICHYCDARFRIRGYLTRHIKKHAKRKAYHCPFFDNSISQELRCHTSGGFSRRDTYKTHLKSRHFTYPEGVKPQDRNKSPGVCTQCGEHFSTSESWVENHIEAGSCKGLPEGYSEGIREKKKTSKMKMIKTSDGQTRFISSDESVSEPALQNKNCIEATVMQSKERPNDKIIPTKTEKNDFGIGTQWFERKQISRPTQTTQSRGPTEVQNLKEWSIISPPILSPQNASSVPQEYQSSRYTLHMDSPALSSASSALSPLSGDPITTTETNKSYPLDSEQSLLEPDKTEEDAINQSKESNMISINEMLQKQMDFELLGENHLKETQDYLALYKKAYGIEF |
| Enzyme Length |
541 |
| Uniprot Accession Number |
P38704 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Transcription factor involved in the regulation of gene expression in response to extracellular amino acid levels. Synthesized as latent cytoplasmic precursor, which, upon a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system, becomes proteolytically activated and relocates to the nucleus, where it induces the expression of SPS-sensor-regulated genes, including the amino-acid permeases BAP2 and BAP3. Binding to promoters is facilitated by DAL81 (By similarity). Involved in the repression of genes subject to nitrogen catabolite repression and genes involved in stress response. Negatively regulated by inner nuclear membrane proteins ASI1, ASI2 and ASI3, which prevent unprocessed precursor forms that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes. {ECO:0000250, ECO:0000269|PubMed:10648791, ECO:0000269|PubMed:15197729, ECO:0000269|PubMed:16735580, ECO:0000269|PubMed:17085444}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Compositional bias (1); Mutagenesis (1); Propeptide (1); Region (4); Sequence conflict (1); Zinc finger (3) |
| Keywords |
Cell membrane;DNA-binding;Membrane;Metal-binding;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger;Zymogen |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Nucleus {ECO:0000269|PubMed:12502738, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:15197729, ECO:0000269|PubMed:16735580}. Note=Localizes to the cytoplasm in its unprocessed form and is targeted to the nucleus after proteolytic processing upon induction by amino acids. |
| Modified Residue |
|
| Post Translational Modification |
PTM: Activated by the amino acid-induced proteolytic removal of an N-terminal inhibitory domain by serine protease SSY5, an intrinsic component of the SPS-sensor. Processing requires at least 2 components of the SCF(GRR1) ubiquitin ligase complex, namely the F-box protein GRR1 and the E2 enzyme CDC34, but does not depend on the proteasome. Processing is negatively regulated by the protein phosphatase 2A regulatory subunit RTS1 (By similarity). {ECO:0000250}. |
| Signal Peptide |
|
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
12702263;
12860974;
14657499;
14690591;
15126393;
15450611;
15699485;
16077013;
16361226;
16420678;
17603098;
17828247;
18708352;
19008939;
19143640;
19805573;
19906648;
20529925;
21127045;
21179020;
21521246;
22189060;
22369481;
22419079;
23144132;
23990766;
24040173;
24483210;
24530295;
25218923;
25253722;
25825491;
26546823;
26760377;
26825800;
27683589;
|
| Motif |
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| Gene Encoded By |
|
| Mass |
60,792 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
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| Cross Reference Brenda |
|