| IED ID | IndEnz0002008908 |
| Enzyme Type ID | protease008908 |
| Protein Name |
Protein P1-P2 Cleaved into: Serine protease EC 3.4.21.- ; RNA-directed RNA polymerase EC 2.7.7.48 69.6 kDa protein |
| Gene Name | ORF1/ORF2 |
| Organism | Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Sobelivirales Solemoviridae Polerovirus Potato leafroll virus (PLrV) Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV) |
| Enzyme Sequence | MNRFTAYAALFFIFSLCSTAKEAGFQHPAFNFRGTSTMSALSGDYSAAPTPLYKSWALPSSLNLTTQPPPLLTDRSYYELVQALISKMRLDCQTVGDMTWRHLSEMLFASWNSVKEVSLKAASVTLWAIISIWFGLYWTLARLITLFLWTFSIEALCLILLGCITSLIYKGALSLSEHLPVFLFMSPLKIIWRAAFSKRNYKNEKAVEGYKGFSVPQKPPKSAVIELQHENGSHLGYANCIRLYSGENALVTAEHCLEGAFATSLKTGNRIPMSTFFPIFKSARNDISILVGPPNWEGLLSVKGAHFITADKIGKGPASFYTLEKGEWMCHSATIDGAHHQFVSVLCNTGPGYSGTGFWSSKNLLGVLKGFPLEEECNYNVMSVIPSIPGITSPNYVFESTAVKGRVFSDETVKELEREASEAVKKLARFKSLTGKNWANDYDSDEDYGLEKEAATNAPAEKTAQTNSAEKTAPSTSAEKTAPTNKPFKWASGTARQNKRQLRHPRRRYKRTTNGQNGRTDHHSYGGENQSLGDRGEDSEQGVSESPAEAQTKQTRKTWREEQAKQFTSYFDAIYKWGAQEEGCPPGFRKCGNIPGYYHPRTKGETKWGQKLCQVHPELADKTAGFGWPKAGFEAELQSLNLQAARWLQRAESATIPGAEARKRVIEKTVEAYRNCITNAPLCSLKSKLDWAGFQQDIREAVQSLELDAGVGIPYIAYGLPTHRGWVEDHKLLPVLTQLTFDRLQKMSEASFEDMSAEELVQEGLCDPIRLFVKGEPHKQSKLDEGRYRLIMSVSLVDQLVARVLFQNQNKREISLWRSVPSKPGFGLSTDTQTAEFLECLQKVSGAPSVEELCANHKEHTRPTDCSGFDWSVAYWMLEDDMEVRNRLTFNNTQLTERLRAAWLKCIGNSVLCLSDGTLLAQTVPGVQKSGSYNTSSSNSRIRVMAAYHCGADWAMAMGDDALEAPNSDLEEYKTLGFKVEVGRELEFCSHIFRNPTLAVPVNTNKMLYKLIHGYNPECGNPEVIQNYLAAVFSVLQELRHDRELVAKLHQWLVPSATTKEH |
| Enzyme Length | 1062 |
| Uniprot Accession Number | P11623 |
| Absorption | |
| Active Site | ACT_SITE 255; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 286; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 354; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.4.21.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: Precursor from which the RNA-dependent RNA polymerase (RdRp) is probably released. RNA-dependent RNA polymerase plays an essential role in virus replication (Potential). {ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (3); Compositional bias (2); Domain (2); Region (1); Sequence caution (1); Signal peptide (1); Site (2); Transmembrane (3) |
| Keywords | Hydrolase;Membrane;Multifunctional enzyme;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Ribosomal frameshifting;Serine protease;Signal;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Protein P1-P2]: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the RdRp (Potential). Cleavages have been shown in the P1 protein, but since the N-terminus containing the serine protease is shared between P1 and P1-P2, cleavages should also occur within the P1-P2 protein. {ECO:0000305}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 118,686 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |