Detail Information for IndEnz0002008915
IED ID IndEnz0002008915
Enzyme Type ID protease008915
Protein Name DEAD-box ATP-dependent RNA helicase 15
EC 3.6.4.13
API5-interacting protein 1
Gene Name AIP2 Os01g0550000 LOC_Os01g36920 B1156H12.21-1 OSJNBa0024F24.6-1
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MGEAEVKDNEVYEEDLVDYEEEVENGADGGAAAANASADVVKKGYVGIHSSGFRDFLLKPELLRAIQDCGFEHPSEVQHECIPQAILGMDVICQAKSGMGKTAVFVLSSLQQIDPVAGQVGALVLCHTRELAYQICHEFERFSKYLPEVKVAVFYGGVHIKKHKDLLKNDCPHIVVGTPGRILALAREKDLSLKNVRHFILDECDKMLDSLDMRRDVQEIFKMTPHDKQVMMFSATLSKEIRPVCKKFMQDPMEIYVDDEAKLTLHGLVQHYIKLSEAEKNRKLNDLLDALDFNQVVIFVKSVSRAAELNKLLCECNFPAISIHSGMTQEERLTRYKNFKEGHKRILVATDLVGRGIDIERVNIVINYDMPDSADSYLHRVGRAGRFGTKGLAITFVSSASDSDVLNQVQERFEVDIKELPEQIDTSTYMPS
Enzyme Length 432
Uniprot Accession Number Q5JK84
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
DNA Binding
EC Number 3.6.4.13
Enzyme Function FUNCTION: ATP-binding RNA helicase involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus (By similarity). Required for tapetal programmed cell death (PCD) and degeneration during anther development. Forms dimer with AIP1 and binds the promoter region of the cysteine protease CP1. Can complement the yeast RNA helicase SUB2. Plants silencing AIP1 and AIP2 are male sterile (PubMed:21467577). {ECO:0000250|UniProtKB:Q07478, ECO:0000269|PubMed:21467577}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 95..102; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00541
Features Chain (1); Coiled coil (1); Domain (2); Motif (2); Nucleotide binding (1)
Keywords ATP-binding;Coiled coil;Helicase;Hydrolase;Nucleotide-binding;Nucleus;RNA-binding;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21467577}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 20878348;
Motif MOTIF 51..79; /note=Q motif; MOTIF 202..205; /note=DEAD box
Gene Encoded By
Mass 48,567
Kinetics
Metal Binding
Rhea ID RHEA:13065
Cross Reference Brenda