| IED ID |
IndEnz0002008944 |
| Enzyme Type ID |
protease008944 |
| Protein Name |
Greglin
|
| Gene Name |
|
| Organism |
Schistocerca gregaria (Desert locust) (Gryllus gregarius) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Protostomia
Ecdysozoa
Panarthropoda
Arthropoda
Mandibulata
Pancrustacea
Hexapoda
Insecta
Dicondylia
Pterygota (winged insects)
Neoptera
Polyneoptera
Orthoptera
Caelifera (grasshoppers and locusts)
Acrididea
Acridomorpha
Acridoidea
Acrididae (short-horned grasshoppers)
Cyrtacanthacridinae (migratory bird locusts)
Schistocerca
Schistocerca gregaria (Desert locust) (Gryllus gregarius)
|
| Enzyme Sequence |
SEDDGSASPESQEMSYTELPCPSICPLIYAPVCVEDSNQDFYLFVNECEVRKCGCEAGFVYTFVPREMCKATTSLCPMQTKSS |
| Enzyme Length |
83 |
| Uniprot Accession Number |
P85064 |
| Absorption |
|
| Active Site |
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| Activity Regulation |
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| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
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| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Serine protease inhibitor (PubMed:16839309, PubMed:23075397). Inhibits porcine pancreatic elastase with a Ki of 58.3 nM, human neutrophil elastase with a Ki of 3.6 nM, cathepsin G with a Ki of 153.5 nM, chymotrypsin with a Ki of 26.7 nM and subtilisin with a Ki of 0.68 nM. Does not inhibit neutrophil protease 3 or pancreatic trypsin (PubMed:16839309). {ECO:0000269|PubMed:16839309, ECO:0000269|PubMed:23075397}. |
| Temperature Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable (PubMed:16839309, PubMed:23075397). No decrease in activity was observed after heating for 1 hour at up to 95 degrees Celsius (PubMed:16839309, PubMed:23075397). {ECO:0000269|PubMed:16839309, ECO:0000269|PubMed:23075397}; |
| PH Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: No decrease in activity observed after incubating at pH 2.5, pH 7.4 and at pH 11.0 for 1 hour to overnight (PubMed:16839309, PubMed:23075397). {ECO:0000269|PubMed:16839309, ECO:0000269|PubMed:23075397}; |
| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (4); Chain (1); Disulfide bond (4); Helix (2); Modified residue (3); Sequence conflict (3) |
| Keywords |
3D-structure;Direct protein sequencing;Disulfide bond;Phosphoprotein;Protease inhibitor;Serine protease inhibitor |
| Interact With |
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| Induction |
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| Subcellular Location |
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| Modified Residue |
MOD_RES 8; /note="Phosphoserine"; /evidence="ECO:0000269|PubMed:16839309, ECO:0000269|PubMed:23075397"; MOD_RES 11; /note="Phosphoserine"; /evidence="ECO:0000269|PubMed:16839309"; MOD_RES 15; /note="Phosphoserine"; /evidence="ECO:0000269|PubMed:16839309" |
| Post Translational Modification |
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| Signal Peptide |
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| Structure 3D |
X-ray crystallography (1) |
| Cross Reference PDB |
4GI3;
|
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
9,195 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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