| IED ID | IndEnz0002008973 |
| Enzyme Type ID | protease008973 |
| Protein Name |
Sodium-dependent dopamine transporter DA transporter DAT Solute carrier family 6 member 3 |
| Gene Name | Slc6a3 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MSKSKCSVGPMSSVVAPAKESNAVGPREVELILVKEQNGVQLTNSTLINPPQTPVEAQERETWSKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPVLKGVGFTVILISFYVGFFYNVIIAWALHYFFSSFTMDLPWIHCNNTWNSPNCSDAHASNSSDGLGLNDTFGTTPAAEYFERGVLHLHQSRGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALLLRGVTLPGAMDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIITTSINSLTSFSSGFVVFSFLGYMAQKHNVPIRDVATDGPGLIFIIYPEAIATLPLSSAWAAVFFLMLLTLGIDSAMGGMESVITGLVDEFQLLHRHRELFTLGIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGVAWFYGVQQFSDDIKQMTGQRPNLYWRLCWKLVSPCFLLYVVVVSIVTFRPPHYGAYIFPDWANALGWIIATSSMAMVPIYATYKFCSLPGSFREKLAYAITPEKDHQLVDRGEVRQFTLRHWLLL |
| Enzyme Length | 619 |
| Uniprot Accession Number | P23977 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Amine transporter (PubMed:1948035, PubMed:1948034, PubMed:1765147, PubMed:1502198). Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (Probable) (PubMed:1765147). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity). {ECO:0000250|UniProtKB:Q61327, ECO:0000269|PubMed:1502198, ECO:0000269|PubMed:1765147, ECO:0000269|PubMed:1948034, ECO:0000269|PubMed:1948035, ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (2); Glycosylation (4); Metal binding (9); Region (1); Sequence conflict (1); Site (1); Topological domain (3); Transmembrane (12) |
| Keywords | Cell membrane;Cell projection;Disulfide bond;Glycoprotein;Membrane;Metal-binding;Neurotransmitter transport;Reference proteome;Sodium;Symport;Transmembrane;Transmembrane helix;Transport |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1502198, ECO:0000269|PubMed:1765147, ECO:0000269|PubMed:1948034, ECO:0000269|PubMed:1948035}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q7K4Y6}. Cell projection, neuron projection {ECO:0000269|PubMed:32963038}. Cell projection, axon {ECO:0000250|UniProtKB:Q01959}. Note=Localizes to neurite tips in neuronal cells (PubMed:32963038). Colocalizes with SEPTIN4 at axon terminals, especially at the varicosities (By similarity). {ECO:0000250|UniProtKB:Q61327, ECO:0000269|PubMed:32963038}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 68,746 |
| Kinetics | |
| Metal Binding | METAL 75; /note=Sodium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 77; /note=Sodium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 78; /note=Sodium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 82; /note=Sodium 2; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 320; /note=Sodium 2; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 352; /note=Sodium 2; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 417; /note=Sodium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 420; /note=Sodium 1; /evidence=ECO:0000250|UniProtKB:Q7K4Y6; METAL 421; /note=Sodium 1; /evidence=ECO:0000250|UniProtKB:Q7K4Y6 |
| Rhea ID | |
| Cross Reference Brenda |