| IED ID | IndEnz0002009055 |
| Enzyme Type ID | protease009055 |
| Protein Name |
Peruvianin-1 EC 3.4.22.- Peruvianin-I Fragment |
| Gene Name | |
| Organism | Thevetia peruviana (Yellow oleander) (Cascabela thevetia) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Gentianales Apocynaceae Rauvolfioideae Plumerieae Thevetiinae Thevetia Thevetia peruviana (Yellow oleander) (Cascabela thevetia) |
| Enzyme Sequence | ADPGPLQDFCLADLNSPLFINGYPCRNPALAISDDF |
| Enzyme Length | 36 |
| Uniprot Accession Number | C0HJY2 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by iodoacetamide and trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane (E-64) but not by phenylmethylsulfonyl fluoride (PMSF), pepstatin-A, ethylenediamine tetra acetic acid (EDTA) or ethylene glycol tetraacetic acid (EGTA). {ECO:0000269|PubMed:26794967}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease able to degrade azocasein and benzoyl-arginine-beta-naphtylamide (BANA) in vitro. {ECO:0000269|PubMed:26794967}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 25-37 degrees Celsius. {ECO:0000269|PubMed:26794967}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5-6. {ECO:0000269|PubMed:26794967}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Direct protein sequencing;Hydrolase;Protease;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:26794967}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 3,881 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=17.6 uM for azocasein (at pH 6 and 37 degrees Celsius) {ECO:0000269|PubMed:26794967}; Vmax=46 nmol/sec/ug enzyme toward azocasein (at pH 6 and 37 degrees Celsius) {ECO:0000269|PubMed:26794967}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |