| IED ID | IndEnz0002009088 |
| Enzyme Type ID | protease009088 |
| Protein Name |
Membrane-bound transcription factor site-2 protease homolog EC 3.4.24.- Endopeptidase S2P |
| Gene Name | S2P At4g20310 F1C12.220 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MEISGRRMRRFRMRFRRDHLTGGENIENEASCCYCDLKISNFNEPIFRLGRRFSGVLKVWFSIGLGFGVASLILVTVFLLLQFHSNPLFSNRLTSAVFGFSPSTRVSLSGIAYVLVSTVITVSVHELGHALAAASEGIQMEYIAVFIAAIFPGGLVAFDNDVLQSLPSFNALRIYCAGIWHNAVFCALCVFALFLLPVMLSPFYKHGESLTVVDVPSVSPLFGYLSPGDVIVSLDGIQVHKPSEWLELAAILDKENSKTSNGSLYLGGSRRFHHGKGYCVPISLIEEGYKGKMVENQFVCPGDLTAFRTMPCSNAAIREVSVCLDAKDIVKLQKCGDGWVTTSDTDNQSDCVCPQGDLCLQAMQSPGVLWTEITYKRTSSQDCSRLGLDFNTSNCLGTFVFVGDLIAMSHSVHLTAYQPRWLFNFFGKSFPNILERSLTCTFHVSLALVLLNSLPVYYLDGESILESSLQSFTWLSPRKKKKALQVCLVGGSLLSFLAFFRIFLLGLPLSRRW |
| Enzyme Length | 513 |
| Uniprot Accession Number | F4JUU5 |
| Absorption | |
| Active Site | ACT_SITE 126; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Metalloprotease that catalyzes the second step (site-2 cleavage) in the proteolytic activation of various factors, after site-1 cleavage. Part of a regulated intramembrane proteolysis (RIP) cascade. After ER stress, cleaves BZIP17 and BZIP28 proteins which functions as stress sensors and transducers in ER stress signaling pathway. The N-terminal bZIP component is translocated to the nucleus, where it activates the expression and production of ER chaperones, as well as proteins involved in brassinosteroid (BR) signaling, which is required for stress acclimation and growth. {ECO:0000269|PubMed:20876872}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous gene model prediction (2); Metal binding (2); Topological domain (7); Transmembrane (6) |
| Keywords | Alternative splicing;Golgi apparatus;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305|PubMed:20876872}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15659096; 17064408; 17662035; 19017746; |
| Motif | |
| Gene Encoded By | |
| Mass | 56,824 |
| Kinetics | |
| Metal Binding | METAL 125; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 129; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.85; |