| IED ID | IndEnz0002009124 |
| Enzyme Type ID | protease009124 |
| Protein Name |
Replicase polyprotein 1TF ORF1ab polyprotein Cleaved into: Nsp1 EC 3.4.22.- ; Nsp1-alpha papain-like cysteine proteinase EC 3.4.22.- PCP1-alpha ; Nsp1-beta papain-like cysteine proteinase EC 3.4.22.- PCP1-beta ; Nsp2TF |
| Gene Name | |
| Organism | Porcine reproductive and respiratory syndrome virus (strain Lelystad) (PRRSV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Nidovirales Arnidovirineae Arteriviridae Variarterivirinae Betaarterivirus Eurpobartevirus Betaarterivirus suid 1 Porcine reproductive and respiratory syndrome virus 1 Porcine reproductive and respiratory syndrome virus (strain Lelystad) (PRRSV) |
| Enzyme Sequence | MSGTFSRCMCTPAARVFWNAGQVFCTRCLSARSLLSPELQDTDLGAVGLFYKPRDKLHWKVPIGIPQVECTPSGCCWLSAVFPLARMTSGNHNFLQRLVKVADVLYRDGCLAPRHLRELQVYERGCNWYPITGPVPGMGLFANSMHVSDQPFPGATHVLTNSPLPQQACRQPFCPFEEAHSSVYRWKKFVVFTDSSLNGRSRMMWTPESDDSAALEVLPPELERQVEILIRSFPAHHPVDLADWELTESPENGFSFNTSHSCGHLVQNPDVFDGKCWLSCFLGQSVEVRCHEEHLADAFGYQTKWGVHGKYLQRRLQVRGIRAVVDPDGPIHVEALSCPQSWIRHLTLDDDVTPGFVRLTSLRIVPNTEPTTSRIFRFGAHKWYGAAGKRARAKRAAKSEKDSAPTPKVALPVPTCGITTYSPPTDGSCGWHVLAAIMNRMINGDFTSPLTQYNRPEDDWASDYDLVQAIQCLRLPATVVRNRACPNAKYLIKLNGVHWEVEVRSGMAPRSLSRECVVGVCSEGCVAPPYPADGLPKRALEALASAYRLPSDCVSSGIADFLANPPPQEFWTLDKMLTSPSPERSGFSSLYKLLLEVVPQKCGATEGAFIYAVERMLKDCPSSKQAMALLAKIKVPSSKAPSVSLDECFPTDVLADFEPASQERPQSSGAAVVLCSPDAKEFEEAAPEEVQESGHKAVHSALLAEGPNNEQVQVVAGEQLKLGGCGLAVGNAHEGALVSAGLINLVGGNLSPSDPMKENMLNSREDEPLDLSQPAPASTTTLVREQTPDNPGSDAGALPVTVREFVPTGPILCHVEHCGTESGDSSSPLDLSDAQTLDQPLNLSLAAWPVRATASDPGWVHGRREPVFVKPRNAFSDGDSALQFGELSESSSVIEFDRTKDAPVVDAPVDLTTSNEALSVVDPFEFAELKRPRFSAQALIDRGGPLADVHAKIKNRVYEQCLQACEPGSRATPATREWLDKMWDRVDMKTWRCTSQFQAGRILASLKFLPDMIQDTPPPVPRKNRASDNAGLKQLVAQWDRKLSVTPPPKPVGPVLDQIVPPPTDIQQEDVTPSDGPPHAPDFPSRVSTGGSWKGLMLSGTRLAGSISQRLMTWVFLKFSPTSQLLCSHFSRRGALWLQVIGCLQVSFYLLSCSVVLTRYSDAFPYWVSFLVLCGVFVWVFLVLGWLLLYFYSRLHPTQSVLLVTTIRRSVMLSFWLLSSANFGNLCAALWSAPQASYVSFLASYSVGHVISGMFSYVYACLQIWPFLLFMWCPRGVVTSVGESV |
| Enzyme Length | 1285 |
| Uniprot Accession Number | P0DJZ9 |
| Absorption | |
| Active Site | ACT_SITE 76; /note="For Nsp1-alpha papain-like cysteine proteinase activity"; /evidence="ECO:0000250|UniProtKB:Q04561, ECO:0000255|PROSITE-ProRule:PRU00872"; ACT_SITE 146; /note="For Nsp1-alpha papain-like cysteine proteinase activity"; /evidence="ECO:0000250|UniProtKB:Q04561, ECO:0000255|PROSITE-ProRule:PRU00872"; ACT_SITE 276; /note="For Nsp1-beta papain-like cysteine proteinase activity"; /evidence="ECO:0000250|UniProtKB:Q04561, ECO:0000255|PROSITE-ProRule:PRU00873"; ACT_SITE 345; /note="For Nsp1-beta papain-like cysteine proteinase activity"; /evidence="ECO:0000250|UniProtKB:Q04561, ECO:0000255|PROSITE-ProRule:PRU00873" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.-; 3.4.22.-; 3.4.22.- |
| Enzyme Function | FUNCTION: [Nsp1-alpha papain-like cysteine proteinase]: Inhibits host IFN-beta production. Plays a role in the degradation of the host transcriptional activator CREBBP protein. The degradation of host CREBBP which is a key component of the IFN enhanceosome is likely responsible for the inhibition of interferon mediated by Nsp1-alpha. Participates also in the inhibition of host NF-kappa-B activation. {ECO:0000250|UniProtKB:Q9WJB2, ECO:0000269|PubMed:23287061}.; FUNCTION: [Nsp1-beta papain-like cysteine proteinase]: Plays a role in the inhibition of the interferon-activated JAK/STAT signal transduction by mediating the ubiquitination and subsequent proteasomal degradation of host KPNA1. {ECO:0000250|UniProtKB:Q9WJB2}.; FUNCTION: [Nsp2TF]: Plays a role in viral replication. {ECO:0000269|PubMed:23043113}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (5); Compositional bias (1); Domain (2); Region (4); Site (2); Transmembrane (4); Zinc finger (1) |
| Keywords | Host cytoplasm;Host membrane;Host nucleus;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Ribosomal frameshifting;Thiol protease;Transmembrane;Transmembrane helix;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Nsp1]: Host nucleus {ECO:0000269|PubMed:23287061}. Host cytoplasm {ECO:0000269|PubMed:23287061}.; SUBCELLULAR LOCATION: [Nsp1-alpha papain-like cysteine proteinase]: Host nucleus {ECO:0000269|PubMed:23287061}. Host cytoplasm {ECO:0000269|PubMed:23287061}.; SUBCELLULAR LOCATION: [Nsp1-beta papain-like cysteine proteinase]: Host nucleus {ECO:0000269|PubMed:23287061}.; SUBCELLULAR LOCATION: [Nsp2TF]: Host cytoplasm {ECO:0000269|PubMed:23043113}. Host membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Note=Nsp2 and nsp2TF localize to different intracellular compartments. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 141,133 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |