| IED ID |
IndEnz0002009177 |
| Enzyme Type ID |
protease009177 |
| Protein Name |
Securin
|
| Gene Name |
PDS1 YDR113C YD9727.08C |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Saccharomycotina (true yeasts)
Saccharomycetes
Saccharomycetales
Saccharomycetaceae
Saccharomyces
Saccharomyces cerevisiae (Baker's yeast)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
|
| Enzyme Sequence |
MMPANEDKENNIVYTGNESSGINFPQTPAHLLKRSHSNILKPPVRLDQLKRDANSNNGNTLKYIQGGKEVSPTKRLHTHAQQQGRLPLAAKDNNRSKSFIFPETSNQSKDADLPQLQNTLSIRKNDQLRKLSQISRSRSRANHNDLLSNSRKLQKYGSVLGYNALPKMKSLVLKDLADSGKNEESSDDDEGNEDSESKLGKKLQSALLKQDSSDGENELNGGLGLFNEQGGLQQLIKNSTKNEQKTKNDKSDKTDDYDIEIAPQRQEPLPYVPEGYSPFQQDDIEKLKTFNSPYKLDLEDEDDTPDKVDLLPLEQIDEEGEKDETECITRNQEEGAALPLLSKNFKEVAAVPTMELVYSEEGLDPEELEDLVT |
| Enzyme Length |
373 |
| Uniprot Accession Number |
P40316 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1. {ECO:0000269|PubMed:9635435}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Compositional bias (2); Helix (3); Modified residue (6); Motif (1); Mutagenesis (4); Region (2) |
| Keywords |
3D-structure;Cell cycle;Cell division;Chromosome partition;Cytoplasm;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation |
| Interact With |
Q03018 |
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. |
| Modified Residue |
MOD_RES 185; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956"; MOD_RES 186; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956"; MOD_RES 212; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956"; MOD_RES 213; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956"; MOD_RES 277; /note="Phosphoserine"; /evidence="ECO:0000305|PubMed:12050115, ECO:0007744|PubMed:19779198"; MOD_RES 292; /note="Phosphoserine; by CDC28"; /evidence="ECO:0000305|PubMed:12050115" |
| Post Translational Modification |
PTM: Phosphorylated by CDC28. The phosphorylation may be important for ESP1 localization to the nucleus. {ECO:0000269|PubMed:12050115}.; PTM: Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. {ECO:0000269|PubMed:8985178}. |
| Signal Peptide |
|
| Structure 3D |
X-ray crystallography (2) |
| Cross Reference PDB |
5U1S;
5U1T;
|
| Mapped Pubmed ID |
10209118;
10322145;
10323869;
10413604;
10421570;
10429181;
10444592;
10444593;
10490625;
10519004;
10550056;
10559897;
10607629;
10632713;
10647015;
10651900;
10654086;
10679389;
10679394;
10679395;
10688190;
10740270;
10747049;
10801468;
10855492;
10949022;
11137013;
11149918;
11163155;
11163156;
11238935;
11283351;
11323716;
11371343;
11390356;
11415980;
11433293;
11448778;
11497275;
11511341;
11566880;
11698195;
11700052;
11702777;
11724813;
11805837;
11917116;
11985612;
12152065;
12220679;
12383796;
12486135;
12598903;
12600308;
12683916;
12717442;
12947083;
14573464;
14660704;
14712073;
15034296;
15187260;
15190205;
15229282;
15533838;
15542824;
15620645;
15649358;
15687272;
15701789;
15703941;
15797376;
15797379;
15797380;
15887295;
15897183;
16455487;
16554755;
16651657;
16713564;
16825537;
16839182;
17006448;
17031663;
17050679;
17130241;
17316440;
17485442;
17502422;
17668044;
17671432;
18081426;
18094053;
18184688;
18454191;
18519644;
18552837;
18585101;
18591250;
18598214;
18616603;
18633407;
18677117;
18680435;
18719252;
18984588;
19001291;
19023403;
19144818;
19268715;
19299562;
19339280;
19464963;
19520826;
19536198;
19767188;
19846659;
19853567;
19893618;
20011546;
20110349;
20407133;
20421994;
20465793;
20600003;
20930506;
21098112;
21483811;
21518961;
21750572;
21806783;
22072716;
22209528;
22361149;
22383977;
22510435;
22675309;
22677545;
22814605;
23028185;
23169651;
23212898;
23468650;
24395824;
24776751;
24930963;
25072887;
25287299;
25300489;
25822502;
26252546;
26332045;
26375390;
26895425;
26994663;
27325700;
27418100;
27450768;
27469264;
27672091;
28146474;
31488515;
32402080;
8601616;
9017389;
9405617;
9482731;
9501986;
9504909;
9763445;
9914173;
9920414;
|
| Motif |
MOTIF 85..88; /note=D-box |
| Gene Encoded By |
|
| Mass |
41,838 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|