| IED ID | IndEnz0002009199 |
| Enzyme Type ID | protease009199 |
| Protein Name |
Transposon Tf2-6 polyprotein Retrotransposable element Tf2 155 kDa protein |
| Gene Name | Tf2-6 SPAC27E2.08 |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MSYANYRYMKARAKRWRPENLDGIQTSDEHLINLFAKILSKHVPEIGKFDPNKDVESYISKLDQHFTEYPSLFPNEHTKRQYTLNHLEELEQQFAERMFSENGSLTWQELLRQTGKVQGSNKGDRLTKTFEGFRNQLDKVQFIRKLMSKANVDDFHTRLFILWMLPYSLRKLKERNYWKSEISEIYDFLEDKRTASYGKTHKRFQPQNKNLGKESLSKKNNTTNSRNLRKTNVSRIEYSSNKFLNHTRKRYEMVLQAELPDFKCSIPCLIDTGAQANIITEETVRAHKLPTRPWSKSVIYGGVYPNKINRKTIKLNISLNGISIKTEFLVVKKFSHPAAISFTTLYDNNIEISSSKHTLSQMNKVSNIVKEPELPDIYKEFKDITAETNTEKLPKPIKGLEFEVELTQENYRLPIRNYPLPPGKMQAMNDEINQGLKSGIIRESKAINACPVMFVPKKEGTLRMVVDYKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTAPAHFQYFINTILGEAKESHVVCYMDDILIHSKSESEHVKHVKDVLQKLKNANLIINQAKCEFHQSQVKFIGYHISEKGFTPCQENIDKVLQWKQPKNRKELRQFLGSVNYLRKFIPKTSQLTHPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNESEPENKRLARWQLFLQDFNFEINYRPGSANHIADALSRIVDETEPIPKDSEDNSINFVNQISITDDFKNQVVTEYTNDTKLLNLLNNEDKRVEENIQLKDGLLINSKDQILLPNDTQLTRTIIKKYHEEGKLIHPGIELLTNIILRRFTWKGIRKQIQEYVQNCHTCQINKSRNHKPYGPLQPIPPSERPWESLSMDFITALPESSGYNALFVVVDRFSKMAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEIVHRYSPALSPLELPSFSDKTDENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQEIEEFQPGDLVMVKRTKTGFLHKSNKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHMFSSTFHVSHLEKYRHNSELNYATIDESDIGTILHILEHKNREQVLYLNVKYISNLNPSTIMSGWTTLATALQADKAIVNDYIKNNNLNI |
| Enzyme Length | 1333 |
| Uniprot Accession Number | P0CT39 |
| Absorption | |
| Active Site | ACT_SITE 271; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Domain (3); Metal binding (5); Region (1) |
| Keywords | Aspartyl protease;DNA integration;DNA recombination;DNA-binding;DNA-directed DNA polymerase;Endonuclease;Hydrolase;Magnesium;Metal-binding;Multifunctional enzyme;Nuclease;Nucleotidyltransferase;Protease;RNA-binding;RNA-directed DNA polymerase;Reference proteome;Transferase;Transposable element |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Processing of the polyproteins proceeds by an ordered pathway, called maturation. It involves the initial cleavage of a 27 kDa capsid protein (CA) from the N-terminus of the polyprotein, followed by the cleavage of a 56 kDa integrase (IN) from the C-terminus. This leaves a 72 kDa protease-reverse transcriptase fusion protein (PR-RT), which does not seem to be processed further (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 154,916 |
| Kinetics | |
| Metal Binding | METAL 502; /note=Magnesium 1; catalytic; for reverse transcriptase activity; /evidence=ECO:0000250; METAL 566; /note=Magnesium 1; catalytic; for reverse transcriptase activity; /evidence=ECO:0000250; METAL 567; /note=Magnesium 1; catalytic; for reverse transcriptase activity; /evidence=ECO:0000250; METAL 990; /note=Magnesium 2; catalytic; for integrase activity; /evidence=ECO:0000250; METAL 1050; /note=Magnesium 2; catalytic; for integrase activity; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |