| IED ID | IndEnz0002009209 |
| Enzyme Type ID | protease009209 |
| Protein Name |
Trypsin EC 3.4.21.4 |
| Gene Name | |
| Organism | Squalus acanthias (Spiny dogfish) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Chondrichthyes Elasmobranchii (elasmobranchs) Selachii (sharks) Squalomorphii Squaliformes Squalidae (dogfish sharks) Squalus Squalus acanthias (Spiny dogfish) |
| Enzyme Sequence | APDDDDKIVGGYECPKHAAPWTVSLNVGYHFCGGSLIAPGWVVSAAHCYQRRIQVRLGEHDISANEGDETYIDSSMVIRHPNYSGYDLDNDIMLIKLSKPAALNRNVDLISLPTGCAYAGEMCLISGWGNTMDGAVSGDQLQCLDAPVLSDAECKGAYPGMITNNMMCVGYMEGGKDSCQGDSGGPVVCNGMLQGIVSWGYGCAERDHPGVYTRVCHYVSWIHETIASV |
| Enzyme Length | 229 |
| Uniprot Accession Number | P00764 |
| Absorption | |
| Active Site | ACT_SITE 47; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 91; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 183; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (6); Domain (1); Metal binding (2); Natural variant (1); Propeptide (1); Site (1) |
| Keywords | Calcium;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Protease;Secreted;Serine protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 24,591 |
| Kinetics | |
| Metal Binding | METAL 59; /note=Calcium; /evidence=ECO:0000250; METAL 69; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |