| IED ID | IndEnz0002009211 |
| Enzyme Type ID | protease009211 |
| Protein Name |
Trypsin EC 3.4.21.4 |
| Gene Name | |
| Organism | Phaedon cochleariae (Mustard beetle) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Coleoptera Polyphaga Cucujiformia Chrysomeloidea Chrysomelidae (leaf beetles) Chrysomelinae Chrysomelini Phaedon Phaedon cochleariae (Mustard beetle) |
| Enzyme Sequence | MIRFTLALAVIGVTFAASTPQIETNPNLEIIGGHDANIIDYPWQISFQHRLHHFCGGFLISDTWVVTAAHCIYEGYSDTENLNIRVGSSEWSAKGKLHDVKRYITHPQYNITTMDNDIALLELALPVDLNQSVRPAKLPVAGQEIPDNAQLTITGWGATYVGGYNEYTLQVVTIPTVNINVCQSAITNDTITNNMFCAGLIGVGGKDSCSGDSGGPAVIDGQVVGIVSWGYSCADPKYPGIYTKVSAFRDWINEETEI |
| Enzyme Length | 258 |
| Uniprot Accession Number | O97399 |
| Absorption | |
| Active Site | ACT_SITE 70; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P35049; ACT_SITE 117; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P35049; ACT_SITE 213; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P35049 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; Evidence={ECO:0000250|UniProtKB:P35049}; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (3); Propeptide (1); Signal peptide (1); Site (1) |
| Keywords | Digestion;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P35049}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,071 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |