| IED ID | IndEnz0002009233 |
| Enzyme Type ID | protease009233 |
| Protein Name |
Ubiquitin thioesterase OTU1 EC 3.4.19.12 OTU domain-containing protein 1 |
| Gene Name | OTU1 YFL044C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MKLKVTGAGINQVVTLKQDATLNDLIEHINVDVKTMRFGYPPQRINLQGEDASLGQTQLDELGINSGEKITIESSDSNESFSLPPPQPKPKRVLKSTEMSIGGSGENVLSVHPVLDDNSCLFHAIAYGIFKQDSVRDLREMVSKEVLNNPVKFNDAILDKPNKDYAQWILKMESWGGAIEIGIISDALAVAIYVVDIDAVKIEKFNEDKFDNYILILFNGIHYDSLTMNEFKTVFNKNQPESDDVLTAALQLASNLKQTGYSFNTHKAQIKCNTCQMTFVGEREVARHAESTGHVDFGQNR |
| Enzyme Length | 301 |
| Uniprot Accession Number | P43558 |
| Absorption | |
| Active Site | ACT_SITE 117; /evidence=ECO:0000250|UniProtKB:Q96FW1; ACT_SITE 120; /note=Nucleophile; /evidence=ECO:0000305; ACT_SITE 222; /evidence=ECO:0000250|UniProtKB:Q5VVQ6; ACT_SITE 294; /evidence=ECO:0000250|UniProtKB:Q96FW1 |
| Activity Regulation | |
| Binding Site | BINDING 221; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q5VVQ6 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q5VVQ6}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquitin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indirectly involved in PIS1 gene expression. {ECO:0000269|PubMed:15755922, ECO:0000269|PubMed:16427015}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Beta strand (11); Binding site (1); Chain (1); Cross-link (1); Domain (1); Helix (9); Mutagenesis (1); Region (5); Turn (6); Zinc finger (1) |
| Keywords | 3D-structure;Cytoplasm;Hydrolase;Isopeptide bond;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 3BY4; 3C0R; 4KDI; 4KDL; |
| Mapped Pubmed ID | 10664582; 11283351; 14690591; 16429126; 16554755; 17142044; 18270205; 18315532; 18719252; 19489724; 19536198; 21741246; 22842922; 23217712; 24610782; 25084388; 25215493; 26503604; 27787227; 9171100; |
| Motif | |
| Gene Encoded By | |
| Mass | 33,510 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |