| IED ID | IndEnz0002009238 |
| Enzyme Type ID | protease009238 |
| Protein Name |
OTU domain-containing protein 5-A EC 3.4.19.12 Deubiquitinating enzyme A DUBA |
| Gene Name | otud5a otud5 si:dkey-83k24.3 si:rp71-30i22.1 zgc:154072 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MTILPKKKPNSSVGVSDHPDDPDRRTGSDPHQHQHQHSHGVRPGARPRASPPPWSYQAAPPASREDRRTESSSRPQQASPPPVGAGSSGGPGDATGMACVSGNRAELSGGVGCGGGMGGCCSGPGLSKRRRQATCSGGVAGGGTGPGAAGGGGGGGGGGGVGGPSPEQEEGAGYNSEDEYENASRLQSEDPATVEQQEHWFEKALQEKKGFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQSGTEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPAFKPGFADQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQPRKASATCSSATAAASSGLEEWNARSPRQRSSAPSPEIPDPAHSDTAAKPPSPAGALALSKPPSPCAPGPSNQACVGPDRPTSSSLVSLYPALGYRAIMQEMSPTAFGLTDWEDDEILASVLAVSQQEYLDSMKKNAMHREPSPDSS |
| Enzyme Length | 560 |
| Uniprot Accession Number | Q08BW0 |
| Absorption | |
| Active Site | ACT_SITE 219; /evidence=ECO:0000255; ACT_SITE 222; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 327; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (1); Compositional bias (2); Domain (1); Erroneous initiation (1); Region (6); Sequence caution (1) |
| Keywords | Alternative splicing;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20040115; |
| Motif | |
| Gene Encoded By | |
| Mass | 60,038 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |