| IED ID | IndEnz0002009241 |
| Enzyme Type ID | protease009241 |
| Protein Name |
OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 OTU domain-containing protein 5 EC 3.4.19.12 Deubiquitinating enzyme OTU5 |
| Gene Name | OTU5 At3g62940 T20O10.40 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MADNKTSEGIVSEETPMDASQEQQHETVEEMLARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDDISKLSTKLKDKQLKELASQGFSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHSKPSKSVKRREKRAKEEADREQRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGHCLYRAVENQLANRSGGASPYTYQNLREMAASYMREHKTDFLPFFLSETEGDSNSGSAEERFEKYCREVESTAAWGSQLELGALTHCLRKHIKVYSGSFPDVEMGKEYRSGDDSSLMLSYHRHAFGLGEHYNSVVLVNNITG |
| Enzyme Length | 332 |
| Uniprot Accession Number | Q9LYC7 |
| Absorption | |
| Active Site | ACT_SITE 189; /evidence=ECO:0000305; ACT_SITE 192; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q8N6M0; ACT_SITE 320; /evidence=ECO:0000250|UniProtKB:Q5VVQ6 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q8N6M0}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation (Probable). Inactive cysteine protease (PubMed:24659992). Deubiquitinating enzyme which regulates gene expression by contributing to chromatin organization and DNA methylation patterns (e.g. H3K4me3 and H3K27me3) (PubMed:29061907). Involved in the interpretation of environmental information, probably by altering chromatin organization and maintaining redox homeostasis (PubMed:29301952). Required for phosphate (Pi) homeostasis (e.g. responses upon Pi starvation including DNA methylation and histone methylation) (PubMed:29061907, PubMed:29301952). Negative regulator of root hair morphogenesis (PubMed:29301952). {ECO:0000269|PubMed:24659992, ECO:0000269|PubMed:29061907, ECO:0000269|PubMed:29301952, ECO:0000305|PubMed:24659992}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (1); Coiled coil (2); Compositional bias (4); Domain (1); Region (1) |
| Keywords | Alternative splicing;Coiled coil;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 32298708; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,362 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |