| IED ID | IndEnz0002009250 |
| Enzyme Type ID | protease009250 |
| Protein Name |
OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 6 OTU domain-containing protein 6 EC 3.4.19.12 Deubiquitinating enzyme OTU6 Otubain-like deubiquitinase 1 |
| Gene Name | OTU6 OTLD1 At2g27350 F12K2.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MTRILVQRGSSGSSSNSSRPSSSSSSSSGSETQINNNIPVPPVTIDEEITDEKQEEVTVVEKAECSDAKDVAVDSDEPADREDDEGLVVAENVHVQSEGIDCDSPVSGGSNSDSPPVPAPPPKPSSTVNPGSNRSVLGSFGALRIGPTRRAAGPRSLVSSRSSPTGSHPSSPRSHSENEGYNSSDEHMPCYVPSHPGSGLEREHQFEAEIRYSKGFEIRRMLEDGNCLFRAVADQVYGDSEAYDLARQMCMDYMEQERDHFSQFITEGFTSYLKRKRRDKVYGNNVEIQALAEMYNRPIHIYSYSTEPINIFQGNYSTDTPPIRLSYHHGNHYNSLVDPHRLTVGAGLGFSSLSGRHVDKEQVKAAIKAQQEHQIDNALLAEGRFYSDLELTEKEIERSVMEASRAEYLMEWSKPRIGPKESSTSNAETSSSGARPSGSDSKPAEAVKEKTVLSSSIEMVLSMGFSYAQAMEAYSIFGDDVDSMVCYVLETSCGGNNRRKGKATE |
| Enzyme Length | 505 |
| Uniprot Accession Number | Q9XIP2 |
| Absorption | |
| Active Site | ACT_SITE 224; /evidence=ECO:0000255; ACT_SITE 227; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q96G74; ACT_SITE 332; /evidence=ECO:0000250|UniProtKB:Q96G74 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:21690391}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation (PubMed:21690391, PubMed:24659992). Binds chromatin (e.g. nucleosomes and histones) and has enzymatic histone deubiquitinase activity, specific for the H2B histone (PubMed:21690391, PubMed:28703681). Can both repress (e.g. OSR2) and promote (e.g. AN3) the expression of target genes by associating with chromatin, deubiquitinating H2B and regulating its euchromatic histone marks (e.g. H3ac and H3K4me) (PubMed:28703681). In association with LDL1/KDM1C, involved in transcriptional gene repression via histone deubiquitination and demethylation (PubMed:21690391). Promotes the concerted epigenetic regulation and repression (e.g. the removal of euchromatic histone acetylation, ubiquitination, and methylation marks) of a set of genes (e.g. GA20OX, WUS, OSR2, ARL and ABI5) that collectively limit plant growth thus stimulating plant growth and increasing cell size (PubMed:27999174). {ECO:0000269|PubMed:21690391, ECO:0000269|PubMed:27999174, ECO:0000269|PubMed:28703681, ECO:0000303|PubMed:24659992}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (1); Compositional bias (4); Domain (2); Region (2) |
| Keywords | Activator;Alternative splicing;Chromatin regulator;Cytoplasm;Hydrolase;Nucleus;Protease;Reference proteome;Repressor;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21690391}. Cytoplasm {ECO:0000269|PubMed:21690391}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12169696; 18775970; 30964380; |
| Motif | |
| Gene Encoded By | |
| Mass | 55,274 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |