| IED ID | IndEnz0002009255 |
| Enzyme Type ID | protease009255 |
| Protein Name |
OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 7 OTU domain-containing protein 7 EC 3.4.19.12 Deubiquitinating enzyme OTU7 |
| Gene Name | OTU7 At5g67170 K21H1.13 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAKTKQQKSKPKKQPHQKQGKDCDLSQFRAQLDALGLKIIQVTADGNCFFRAIADQLEGNEDEHNKYRNMIVLYIVKNREMFEPFIEDDVPFEDYCKTMDDDGTWAGNMELQAASLVTRSNICIHRNMSPRWYIRNFEDTRTRMIHLSYHDGEHYNSVRSKEDACGGPARPVVIEADAKVSAASKQAKATESKSKNKADKCHVNAGAIKVVMSGSCCDNTEKAEQVLLQVNGDVDAAIEFLIADQGMESLTENDTETASASDTINPKHASDSPMENTEQAREELIEEESASGNNSETVQAKCTTQTDDKKIPRNKTCPCGSKKKYKSCCGTATGRSSVKLLVSQTMESKKGRKNLRRGTSNEVEANAPDVGALCI |
| Enzyme Length | 375 |
| Uniprot Accession Number | F4K3M6 |
| Absorption | |
| Active Site | ACT_SITE 45; /evidence=ECO:0000305; ACT_SITE 48; /note=Nucleophile; /evidence=ECO:0000269|PubMed:24659992; ACT_SITE 154; /evidence=ECO:0000250|UniProtKB:Q96G74 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:24659992}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation (PubMed:24659992). Cysteine protease with a preference for 'Lys-63' over 'Lys-48' over 'Met-1' -linked ubiquitin (UB) tetramers as substrates (PubMed:24659992). Cleaves also RUB-GST fusion (PubMed:24659992). {ECO:0000269|PubMed:24659992}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:24659992}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (3); Chain (1); Compositional bias (4); Domain (2); Erroneous gene model prediction (1); Motif (1); Mutagenesis (1); Region (2); Sequence conflict (2) |
| Keywords | Alternative splicing;Hydrolase;Nucleus;Protease;Reference proteome;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 308..315; /note=Nuclear localization signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00768 |
| Gene Encoded By | |
| Mass | 41,534 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |