| IED ID | IndEnz0002009263 |
| Enzyme Type ID | protease009263 |
| Protein Name |
Ubiquitin thioesterase otubain-like EC 3.4.19.12 Deubiquitinating enzyme otubain-like Ubiquitin-specific-processing protease otubain-like |
| Gene Name | otub-1 C25D7.8 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MANEPQKSDDNGQAAEAVVTDDEIVLQDQQLKTIEDEQKSVPLVATLAPFSILCAEYDNETSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKFIASSRDWTRTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHTEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGWHYDIPSDDQQIAPEITLLYRPGHYDVIYKKDSTEASEIEN |
| Enzyme Length | 284 |
| Uniprot Accession Number | Q9XVR6 |
| Absorption | |
| Active Site | ACT_SITE 85; /evidence=ECO:0000250|UniProtKB:Q96DC9; ACT_SITE 88; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q96DC9; ACT_SITE 245; /evidence=ECO:0000250|UniProtKB:Q96FW1; ACT_SITE 267; /evidence=ECO:0000250|UniProtKB:Q96DC9 |
| Activity Regulation | |
| Binding Site | BINDING 176; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q5VVQ6 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q96DC9}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Specifically cleaves 'Lys-48'-linked polyubiquitin. {ECO:0000269|PubMed:19211026}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Beta strand (6); Binding site (1); Chain (1); Domain (1); Helix (14); Turn (1) |
| Keywords | 3D-structure;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | P61088 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 4DHI; 4DHJ; 4DHZ; 4LDT; |
| Mapped Pubmed ID | 14704431; 17612406; 21177967; 21367940; 21611156; 22286215; 22367539; 22560298; 23800452; 23955022; 25487147; 30140741; |
| Motif | |
| Gene Encoded By | |
| Mass | 32,293 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |