| IED ID | IndEnz0002009267 |
| Enzyme Type ID | protease009267 |
| Protein Name |
OTU domain-containing protein 3 EC 3.4.19.12 |
| Gene Name | OTUD3 KIAA0459 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MSRKQAAKSRPGSGSRKAEAERKRDERAARRALAKERRNRPESGGGGGCEEEFVSFANQLQALGLKLREVPGDGNCLFRALGDQLEGHSRNHLKHRQETVDYMIKQREDFEPFVEDDIPFEKHVASLAKPGTFAGNDAIVAFARNHQLNVVIHQLNAPLWQIRGTEKSSVRELHIAYRYGEHYDSVRRINDNSEAPAHLQTDFQMLHQDESNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNAEENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQWMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI |
| Enzyme Length | 398 |
| Uniprot Accession Number | Q5T2D3 |
| Absorption | |
| Active Site | ACT_SITE 73; /evidence=ECO:0000250; ACT_SITE 76; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 182; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:23827681}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains. {ECO:0000269|PubMed:23827681}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (6); Chain (1); Compositional bias (3); Domain (2); Erroneous initiation (1); Helix (7); Modified residue (1); Mutagenesis (1); Natural variant (2); Region (5); Turn (1) |
| Keywords | 3D-structure;Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | Q9BPX1; P60484-1 |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 224; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:23186163 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 4BOU; |
| Mapped Pubmed ID | 19122664; 19915572; 20228798; 20228799; 23511034; 24270891; 26280536; 31266968; 31338872; 32483383; 32571254; 32679077; 32775453; 34380780; 34853315; |
| Motif | |
| Gene Encoded By | |
| Mass | 45,124 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |