| IED ID | IndEnz0002009275 |
| Enzyme Type ID | protease009275 |
| Protein Name |
Papain EC 3.4.22.2 Papaya proteinase I PPI allergen Car p 1 |
| Gene Name | |
| Organism | Carica papaya (Papaya) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Caricaceae Carica Carica papaya (Papaya) |
| Enzyme Sequence | MAMIPSISKLLFVAICLFVYMGLSFGDFSIVGYSQNDLTSTERLIQLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKKNNSYWLGLNVFADMSNDEFKEKYTGSIAGNYTTTELSYEEVLNDGDVNIPEYVDWRQKGAVTPVKNQGSCGSCWAFSAVVTIEGIIKIRTGNLNEYSEQELLDCDRRSYGCNGGYPWSALQLVAQYGIHYRNTYPYEGVQRYCRSREKGPYAAKTDGVRQVQPYNEGALLYSIANQPVSVVLEAAGKDFQLYRGGIFVGPCGNKVDHAVAAVGYGPNYILIKNSWGTGWGENGYIRIKRGTGNSYGVCGLYTSSFYPVKN |
| Enzyme Length | 345 |
| Uniprot Accession Number | P00784 |
| Absorption | |
| Active Site | ACT_SITE 158; /evidence=ECO:0000269|PubMed:5681232; ACT_SITE 292; ACT_SITE 308; /evidence=ECO:0000269|PubMed:5681232 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.; EC=3.4.22.2; |
| DNA Binding | |
| EC Number | 3.4.22.2 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (14); Chain (1); Disulfide bond (3); Helix (11); Propeptide (1); Sequence conflict (4); Signal peptide (1); Turn (5) |
| Keywords | 3D-structure;Allergen;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Signal;Thiol protease;Zymogen |
| Interact With | P01040 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (29) |
| Cross Reference PDB | 1BP4; 1BQI; 1CVZ; 1KHP; 1KHQ; 1PAD; 1PE6; 1PIP; 1POP; 1PPD; 1PPN; 1PPP; 1STF; 2CIO; 2PAD; 3E1Z; 3IMA; 3LFY; 3TNX; 3USV; 4PAD; 4QRG; 4QRV; 4QRX; 5PAD; 6H8T; 6PAD; 6TCX; 9PAP; |
| Mapped Pubmed ID | 10600517; 1445241; 15357669; 16754967; 1860874; 19143838; 21416241; 21543879; 23151624; 30175357; 8416808; 952885; 9804696; |
| Motif | |
| Gene Encoded By | |
| Mass | 38,922 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.2; |