| IED ID | IndEnz0002009312 |
| Enzyme Type ID | protease009312 |
| Protein Name |
26S proteasome regulatory subunit 4 homolog A 26S proteasome AAA-ATPase subunit RPT2a 26S proteasome subunit 4 homolog A Protein HALTED ROOT Regulatory particle triple-A ATPase subunit 2a |
| Gene Name | RPT2A HLR At4g29040 F19B15.70 F25O24.6 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGQGPSGGLNRQGDRKPDGGDKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVTPSTKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSKMTLSEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHPDFKKAKEKVMFKKKEGVPEGLYM |
| Enzyme Length | 443 |
| Uniprot Accession Number | Q9SZD4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (PubMed:22158466) (Probable). Interacts with transit peptides of proteins targeted to the chloroplast, and may be involved in the degradation of unimported plastid protein precursors (PubMed:24846764). Is required for the maintenance of postembryonic root and shoot meristems (PubMed:15073153, PubMed:21784786). Has a specific role in the regulation of organs size (PubMed:19500299, PubMed:19321709). Acts redundantly with RPT2B in the regulation of gametogenesis (PubMed:21784786, PubMed:22158466). With RPT2B plays a critical role in 26S proteasome assembly (PubMed:22158466). Acts as an upstream signaling component for inducing both defense and morphological phenotypes in the constitutive active uni-1D mutant (PubMed:21791544). Acts as negative regulator of endoreduplication in trichome cells (PubMed:20195883). May function after the completion of the third endoreduplication step (8C to 16C) mediated by RHL1 (PubMed:20195883). Acts as negative regulator of transcriptional gene silencing (TGS) at specific endogenous genes through DNA methylation (PubMed:22615900). Promotes post-transcriptional gene silencing (PTGS) by limiting the degradation of transgene aberrant RNAs by the RNA quality control (RQC) machinery, thus favoring their entry into cytoplasmic siRNA bodies where they can trigger PTGS (PubMed:22615900). Involved in tolerance to zinc deficiency, possibly through alleviation of oxidative stresses or processing of poly-ubiquitinated proteins (PubMed:21389614). Required for resistance to the fungal pathogen Golovinomyces cichoracearum (PubMed:22577987). {ECO:0000269|PubMed:15073153, ECO:0000269|PubMed:19321709, ECO:0000269|PubMed:19500299, ECO:0000269|PubMed:20195883, ECO:0000269|PubMed:21389614, ECO:0000269|PubMed:21784786, ECO:0000269|PubMed:21791544, ECO:0000269|PubMed:22158466, ECO:0000269|PubMed:22577987, ECO:0000269|PubMed:22615900, ECO:0000269|PubMed:24846764, ECO:0000305|PubMed:17971041}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 229..236; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Compositional bias (2); Cross-link (2); Nucleotide binding (1); Region (2); Sequence conflict (1) |
| Keywords | ATP-binding;Cytoplasm;Immunity;Innate immunity;Isopeptide bond;Nucleotide-binding;Nucleus;Proteasome;Reference proteome;Stress response;Ubl conjugation |
| Interact With | |
| Induction | INDUCTION: Induced by treatment with the proteasome inhibitor MG132. {ECO:0000269|PubMed:22158466}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000269|PubMed:22615900}. Nucleus {ECO:0000269|PubMed:22615900}. Note=Localizes to siRNA-bodies in the cytoplasm. {ECO:0000269|PubMed:22615900}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10363660; 11742986; 12912986; 15911562; 16400686; 16502469; 16719932; 17432890; 17825468; 18642946; 18650403; 19704803; 20087596; 21166475; 21490420; 22323770; 27613851; 29487187; 30824010; 31740770; 32502259; 32753431; 9741626; |
| Motif | |
| Gene Encoded By | |
| Mass | 49,370 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |