| IED ID | IndEnz0002009323 |
| Enzyme Type ID | protease009323 |
| Protein Name |
Small ubiquitin-related modifier 1 SUMO-1 GAP-modifying protein 1 GMP1 SMT3 homolog 3 Sentrin Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein SMT3C Smt3C Ubiquitin-like protein UBL1 |
| Gene Name | SUMO1 SMT3C SMT3H3 UBL1 OK/SW-cl.43 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV |
| Enzyme Length | 101 |
| Uniprot Accession Number | P63165 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed:24651376). {ECO:0000269|PubMed:18408734, ECO:0000269|PubMed:18538659, ECO:0000269|PubMed:19223394, ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:24651376, ECO:0000269|PubMed:9019411, ECO:0000269|PubMed:9162015}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Beta strand (9); Chain (1); Cross-link (11); Domain (1); Helix (3); Initiator methionine (1); Modified residue (4); Mutagenesis (2); Propeptide (1); Sequence conflict (1); Site (1); Turn (2) |
| Keywords | 3D-structure;Acetylation;Alternative splicing;Cell membrane;Chromosomal rearrangement;Cytoplasm;Host-virus interaction;Isopeptide bond;Membrane;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation;Ubl conjugation pathway |
| Interact With | P10275; P15336; A0A0S2Z4M1; O15169; P38398; Q9Y2F9; Q96B23; Q9H257; O00257; P17676-1; Q9UER7; Q05D60; Q9UBC3; Q86UW9; Q8WWZ3; P06730; P19419; P19419-1; A0A0S2Z3N6; Q9BPY3; Q8TES7-6; Q53SE7; O95073; O14964; Q16665; P07910; Q00613; P42858; Q9Y6K9; P10914; O00180; Q8NDC0; Q9UIS9; Q02078-1; P10242; Q00653; P06748-1; P09874; O75928; O75928-2; P29590; O75626-2; O75360; O75475; Q9Y4B4; P46060; P10276; Q9BQY4; P78317; Q9Y3V2; O43290; Q01826; Q9P0U3; Q9HC62; P56693; P48431; P23497; P23497-2; G2XKQ0; Q92844; Q13569; Q12800; P04637; Q12888-1; Q12933; Q9BUZ4; O00463; Q9UBT2; P63279; Q7KZS0; Q5W0Q7; Q8N680; Q9HCK0; Q15916; Q6PEW1; Q8N3Z6; Q96MM3; Q9UJ78; Q59GP6; Q60636 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:10574707, ECO:0000269|PubMed:12383504}. Nucleus speckle {ECO:0000250|UniProtKB:P63166}. Cytoplasm {ECO:0000269|PubMed:12383504, ECO:0000269|PubMed:9162015}. Nucleus, PML body {ECO:0000269|PubMed:10574707, ECO:0000269|PubMed:12383504, ECO:0000269|PubMed:22406621}. Cell membrane {ECO:0000269|PubMed:19223394}. Nucleus {ECO:0000269|PubMed:24651376, ECO:0000269|PubMed:9162015}. Note=Recruited by BCL11A into the nuclear body (By similarity). In the presence of ZFHX3, sequesterd to nuclear body (NB)-like dots in the nucleus some of which overlap or closely associate with PML body (PubMed:24651376). {ECO:0000250|UniProtKB:P63166, ECO:0000269|PubMed:24651376}. |
| Modified Residue | MOD_RES 2; /note="N-acetylserine"; /evidence="ECO:0000269|PubMed:20388717, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692"; MOD_RES 2; /note="Phosphoserine"; /evidence="ECO:0000269|PubMed:18707152, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163"; MOD_RES 9; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:20068231"; MOD_RES 32; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163" |
| Post Translational Modification | PTM: Cleavage of precursor form by SENP1 or SENP2 is necessary for function. {ECO:0000269|PubMed:15487983}.; PTM: Polymeric SUMO1 chains undergo polyubiquitination by RNF4. {ECO:0000269|PubMed:18408734}. |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (13); X-ray crystallography (44) |
| Cross Reference PDB | 1A5R; 1TGZ; 1WYW; 1Y8R; 1Z5S; 2ASQ; 2BF8; 2G4D; 2IO2; 2IY0; 2IY1; 2KQS; 2LAS; 2MW5; 2N1A; 2N1V; 2PE6; 2UYZ; 2VRR; 3KYC; 3KYD; 3RZW; 3UIP; 4WJN; 4WJO; 4WJP; 4WJQ; 5AEK; 5B7A; 5ELJ; 5GHD; 6EOP; 6EOT; 6J4I; 6JXU; 6JXV; 6K5T; 6TRW; 6UYO; 6UYP; 6UYQ; 6UYR; 6UYS; 6UYT; 6UYU; 6UYV; 6UYX; 6UYY; 6UYZ; 6V7P; 6V7Q; 6V7R; 6V7S; 6WW3; 6XOG; 6XOH; 6XOI; |
| Mapped Pubmed ID | 10187858; 10550055; 10724175; 10759568; 10805787; 10862613; 10866324; 10871618; 10973490; 11025670; 11112409; 11114888; 11121022; 11124955; 11231159; 11477070; 11493912; 11604075; 11709553; 11735126; 11792325; 11842083; 11889051; 11896061; 11948183; 11960997; 12032081; 12060666; 12072434; 12077349; 12144530; 12149243; 12150977; 12177000; 12192048; 12196509; 12200128; 12297306; 12354770; 12356736; 12364621; 12393906; 12419227; 12447390; 12511558; 12529333; 12552083; 12641448; 12646186; 12665592; 12679040; 12697768; 12718889; 12773400; 12788062; 12832072; 12855578; 12947387; 14500761; 14514699; 14516784; 14527952; 14563852; 14578449; 14637113; 14651848; 14752048; 14992729; 15016812; 15037602; 15082759; 15123625; 15192080; 15192092; 15199155; 15220454; 15229220; 15229330; 15247228; 15272016; 15337742; 15355965; 15355988; 15378033; 15456902; 15468306; 15494207; 15507114; 15507434; 15574327; 15592428; 15637059; 15723659; 15766567; 15788563; 15823533; 15831457; 15848177; 15857832; 15881673; 15882793; 15896780; 15907800; 15923626; 15931174; 15958389; 15961505; 15976810; 15989970; 16030353; 16055710; 16098147; 16112644; 16117725; 16120648; 16122737; 16125395; 16127449; 16150728; 16154161; 16169070; 16189514; 16194093; 16253240; 16287980; 16352666; 16415059; 16421094; 16428803; 16460827; 16464864; 16475184; 16478998; 16494873; 16501610; 16563226; 16568089; 16631117; 16651405; 16651613; 16688858; 16738331; 16757475; 16763556; 16781829; 16810316; 16904644; 16906147; 16912044; 17000644; 17018294; 17020914; 17027752; 17036045; 17060459; 17066076; 17079232; 17081986; 17099700; 17105732; 17164289; 17187077; 17283066; 17298944; 17314004; 17320104; 17353931; 17360386; 17439403; 17466333; 17491593; 17509614; 17525340; 17535915; 17553757; 17591783; 17643372; 17671677; 17693435; 17717077; 17803295; 17932034; 17942393; 17976381; 18001824; 18001825; 18006705; 18031230; 18031232; 18063693; 18077395; 18082607; 18093978; 18211901; 18339323; 18374648; 18394993; 18404132; 18408014; 18488044; 18579533; 18592385; 18617891; 18617892; 18625722; 18628979; 18660489; 18682553; 18691969; 18762900; 18832723; 18836734; 18946085; 18948102; 18978678; 18983974; 19001859; 19029252; 19041634; 19057511; 19107418; 19176521; 19203578; 19203579; 19211567; 19214191; 19251700; 19261749; 19287951; 19297320; 19380586; 19384898; 19407830; 19443651; 19450230; 19497852; 19565496; 19596686; 19615980; 19622798; 19625249; 19680224; 19692572; 19744555; 19779455; 19859084; 19864602; 19889771; 19913121; 19917317; 19937600; 19955185; 19995069; 20006587; 20016594; 20016603; 20023648; 20098713; 20145208; 20159957; 20164921; 20176810; 20188669; 20190268; 20209145; 20224576; 20300531; 20351170; 20360068; 20417598; 20467437; 20501649; 20519406; 20533263; 20547755; 20555024; 20584900; 20628086; 20634891; 20661221; 20663916; 20676127; 20711444; 20711500; 20738159; 20797632; 20802522; 20844582; 20881090; 20932933; 20972456; 21039605; 21044801; 21047957; 21057547; 21098080; 21123177; 21167178; 21189065; 21192925; 21212807; 21242980; 21252943; 21268066; 21282113; 21284855; 21293379; 21317888; 21336309; 21338522; 21375726; 21467194; 21474068; 21490953; 21516116; 21527249; 21527745; 21545853; 21550342; 21554500; 21563299; 21641618; 21647297; 21683690; 21722636; 21811235; 21878624; 21880768; 21931855; 21957283; 21968017; 21983723; 21988832; 22022399; 22147707; 22164242; 22179609; 22194619; 22198408; 22227369; 22370726; 22373579; 22388734; 22402492; 22403398; 22457066; 22458338; 22464730; 22467880; 22493095; 22508508; 22555612; 22586270; 22589545; 22612509; 22635276; 22649767; 22651256; 22661229; 22661230; 22685230; 22713328; 22713753; 22731716; 22748127; 22751501; 22878415; 22905217; 22930759; 22975420; 22980979; 23077236; 23078246; 23092970; 23152500; 23152501; 23160374; 23229893; 23243001; 23306437; 23333306; 23350884; 23382880; 23395904; 23404503; 23407422; 23414517; 23417673; 23546875; 23673342; 23727112; 23751493; 23871147; 23996296; 24002598; 24068740; 24072711; 24174529; 24244371; 24286314; 24309115; 24344134; 24390485; 24434214; 24656128; 24667604; 24753249; 24819975; 24835590; 24942926; 24953629; 25074923; 25111678; 25114211; 25118297; 25183729; 25241153; 25378699; 25391294; 25416956; 25484073; 25497731; 25512557; 25704882; 25800734; 25867063; 26042670; 26060329; 26151477; 26212320; 26223657; 26244656; 26400283; 26403314; 26449956; 26496610; 26549688; 26563097; 26578773; 26850942; 26867680; 26993772; 27084229; 27129204; 27195426; 27230680; 27576863; 27799292; 27935866; 28055018; 28222440; 28582471; 28770955; 29138295; 29138839; 29352251; 29382749; 29393359; 29588524; 29634367; 29777712; 30184152; 30355233; 30393970; 30439429; 30562482; 30681271; 30824543; 30926672; 31125786; 31371453; 31476572; 31489957; 31578236; 31587348; 31796536; 31879127; 31914638; 32001251; 32348746; 32413267; 32483411; 32592588; 32706448; 32726574; 32783654; 32860951; 33181633; 33213505; 33326746; 33631934; 34188616; 34247369; 34274315; 34310804; 34663464; 9412458; 9452416; 9497385; 9512523; 9734360; 9756909; 9885291; 9920803; |
| Motif | |
| Gene Encoded By | |
| Mass | 11,557 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |