| IED ID | IndEnz0002009330 |
| Enzyme Type ID | protease009330 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 36 EC 3.4.19.12 Deubiquitinating enzyme 36 Protein scrawny Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
| Gene Name | Usp36 scny GA18934 |
| Organism | Drosophila pseudoobscura pseudoobscura (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora obscura group pseudoobscura subgroup Drosophila pseudoobscura Drosophila pseudoobscura pseudoobscura (Fruit fly) |
| Enzyme Sequence | MPVSMAVCETANVVNAALRESLGVGNGSRAADEAKKTGGGGGDDSDSEMHNQIAVSAYAKRILMSKIEYEEVPNYHDSVLEQLKNKYIVIKQPSNNNNSSSCNGSNFGNSKVVGANGHDNGNNGHRKLTQSESTQSGPSPNELPKPKRVLYPRENIRIGWKQSERKWQVGTGMINVGNTCYLNSTLQALFHIPALANWLVSEQTHLENCNITESNGGCIICAMAKTLQSTQSCQSAMRPFHIYSKLKQICKHMVVGRQEDAHEFLRFLVEAMEKAYLMRFRNFKELDQLVKETTPLNQIFGGYLRSEVRCLSCSHVSITFQHFQDLLLDIRKSDTLEEAFDGYFSRERLEDMGYKCEGCKKKVSATKQFSLERAPITLCIQLKRFSMIGNKLTKTISFKPRIDLSRFAARSPAAAAQPLTYRLVSMVTHLGVSQHCGHYTAIGMTESGSYYNFDDSYVRPIAMQSVCNTNAYIMFYELDLSQTTPLKSNGLRLTNGHSQVAVPATVSSSSPTHTRFIGPQLPPGGINGYSNGHATGSSNAQKTAIQFKQQQQHPQQNGLQVGTGKFQEPPHAKSPLAGAYNKGEAFPATTANGNKSSSSSASNSNHNKSVNQLQHQQHYLPISSEDEDSEDGATATATATATARPTAQLPSMPKMTEDSSDKPKTPLKSSVKTNLVKSLLKTPLKSLVPYESASEEDEPLPNPRKRRSDSDSNDSGDSDPQPGHVNGHTKTNGGSLTNGNGLGKAKTILATSSSSSLASASASAASDDEDADEEEENSKLTNGWQPQKQSQSLTQSKAPPSPKTPPSPAVIKSKTGIWKVTRNDDNEDEDDDDDEDEEEQHQVVSTPSKNPRNPFAKSSTTPGAKRQKLLNGIAVKSQQQPRVGNGYQSEASTNGNVVNELLKQTHRGYGSASVLSWSGKPAELEKELVAEAREQRQHDHDDEEENEMDRGRQRKVKSATAKAYNSSTPGYNPFQEYESQKRWHKSSNGGGSFPRYHNQNFRQNFQQRNKFKYNRFGGPGGAKFQQQRALQRHLASGGVFNRRQPTGQQQQQQSQQSSS |
| Enzyme Length | 1059 |
| Uniprot Accession Number | Q2LZB1 |
| Absorption | |
| Active Site | ACT_SITE 180; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 438; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Controls selective autophagy activation by ubiquitinated proteins. {ECO:0000250|UniProtKB:Q9VRP5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (13); Domain (1); Modified residue (12); Region (6) |
| Keywords | Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. |
| Modified Residue | MOD_RES 508; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 510; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 673; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 682; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 692; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 694; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 766; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 801; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 804; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 807; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 846; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 861; /note=Phosphothreonine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 116,372 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |