| IED ID | IndEnz0002009387 |
| Enzyme Type ID | protease009387 |
| Protein Name |
Serine protease 1 EC 3.4.21.- Glutamic acid-specific protease SFase-1 |
| Gene Name | |
| Organism | Streptomyces fradiae (Streptomyces roseoflavus) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces fradiae (Streptomyces roseoflavus) |
| Enzyme Sequence | MRRTTRARTGLSALLLAASLGLGAAPAGADAPQRPAPTPASDSAAALHALDAAVERTLGDDSAGTYVDAGTGELVVTVTTEAAAAKVRAAGATPRRVQRGAAELDAAMAALEARAKIPGTSWGLDPRTNRIAVEADSSVSARDLARLRKVAASLDGAVSVTRVPGVFQREVAGGDAIYGGGSRCSAAFNVTKNGVRYFLTAGHCTNLSSTWSSTSGGTSIGVREGTSFPTNDYGIVRYTTTTNVDGRVNLYNGGYQDIASAADAVVGQAIKKSGSTTKVTSGTVSAVNVTVNYSDGPVYGMVRTTACSAGGDSGGAHFAGSVALGIHSGSSGCTGTNGSAIHQPVREALSAYGVNVY |
| Enzyme Length | 357 |
| Uniprot Accession Number | Q03424 |
| Absorption | |
| Active Site | ACT_SITE 203; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 232; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 313; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease that preferentially cleaves peptide bonds on the C-terminal side of aspartate and glutamate with a 10-fold higher reactivity for a glutamyl bond than an aspartyl bond. {ECO:0000269|PubMed:8490047}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Propeptide (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 35,849 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |