| IED ID |
IndEnz0002009445 |
| Enzyme Type ID |
protease009445 |
| Protein Name |
Spermatogenesis-associated protein 2
|
| Gene Name |
Spata2 Pd1 |
| Organism |
Rattus norvegicus (Rat) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Sarcopterygii
Dipnotetrapodomorpha
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Boreoeutheria
Euarchontoglires
Glires (Rodents and rabbits)
Rodentia
Myomorpha (mice and others)
Muroidea
Muridae
Murinae
Rattus
Rattus norvegicus (Rat)
|
| Enzyme Sequence |
MDTKYKDDLFRKYVQFHEGKVDTTPGKQSGSDEYLRVAAATLLSLHKVDPLYRFRLIQFYEVVESSLRSLSSSSLSALHCAFSMLETMAINLFLFPWKKEFRSIKTYTGPFVYYVKSTLLEKDIRAILKFMGYEPELGTAYKLKELVESLQVKMVSFELFLAKVECEQMLGIHSQVKDKGYSELDVVTERKSSTEDARGCSDALRRRAESREHLTTSMARVALQKSASERAAKDYYKPRVTKPSRSVDAYDSYWESRKPPSKASLSLRKEPLAMDVGEDLKDEIIRPSPSLLTMSSSPHGSPDDLPSISPINGLGLLRSTYFPTQDDVDLYTDSEPRATYRRQDALRSDIWLVKNDAHSIYHKRSPPTKESALSKCQNCGLSCSSSLCQRCDSVCPSASKPSGFPSKASAHDSLVHGAPMREKYVGQTQGLDRLATVHSKPKPSTTATSRCGFCNRAGATNTCTQCSKVSCDPCLGAYHYDPCCRKSELHKFMPNNQLNYKSTQFSHPVYR |
| Enzyme Length |
511 |
| Uniprot Accession Number |
Q66HP6 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Bridging factor that mediates the recruitment of CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis. Acts as a direct binding intermediate that bridges RNF31/HOIP, the catalytic subunit of the LUBAC complex, and the deubiquitinase (CYLD), thereby recruiting CYLD to the TNF-R1 signaling complex (TNF-RSC). Required to activate the 'Met-1'- (linear) and 'Lys-63'-linked deubiquitinase activities of CYLD (By similarity). Controls the kinase activity of RIPK1 and TNF-alpha-induced necroptosis by promoting 'Met-1'-linked deubiquitination of RIPK1 by CYLD (By similarity). {ECO:0000250|UniProtKB:Q8K004, ECO:0000250|UniProtKB:Q9UM82}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Domain (1); Erroneous initiation (1); Motif (1); Sequence conflict (3) |
| Keywords |
Cytoplasm;Necrosis;Nucleus;Reference proteome |
| Interact With |
|
| Induction |
INDUCTION: Up-regulated following FSH treatment. {ECO:0000269|PubMed:11322771}. |
| Subcellular Location |
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9UM82}. Nucleus {ECO:0000269|PubMed:11322771}. |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
|
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
MOTIF 320..337; /note=PIM motif; /evidence=ECO:0000250|UniProtKB:Q9UM82 |
| Gene Encoded By |
|
| Mass |
57,481 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|