| IED ID | IndEnz0002009515 |
| Enzyme Type ID | protease009515 |
| Protein Name |
Subtilisin-like protease 1 EC 3.4.21.- Destructin-2 Serine protease 1 PdSP1 |
| Gene Name | SP1 GMDG_08491 |
| Organism | Pseudogymnoascus destructans (strain ATCC MYA-4855 / 20631-21) (Bat white-nose syndrome fungus) (Geomyces destructans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Leotiomycetes Leotiomycetes incertae sedis Pseudeurotiaceae Pseudogymnoascus Pseudogymnoascus destructans Pseudogymnoascus destructans (strain ATCC MYA-4855 / 20631-21) (Bat white-nose syndrome fungus) (Geomyces destructans) |
| Enzyme Sequence | MKFSQSLIALAACFLPLIAAAPVEAQHAKIRSPRAQDIIPDSYIVVFNKGVNDADIESEFSSVSRILSKRRSAHKGVGHKYNITGFKGYQIETDTGSIGEIAASPLVAWIEMDGKVQANALETRSGATWGLGRISHKATGSNSYIYDGSAGSGSTVYVLDTGIYIEHSEFEGRAKWGANYISGSPDTDENGHGTHCAGTIAGATYGVASKANLVAVKVLDRDGFGATSATIAGINFVGQNGKDGKSVISMSLRGHYSAAVNSAVESTVSNGVTIVVAAGNDGDDASNYSPASAKNAITVGSVDSTDTRASSSNYGSVVDIFAPGVNVKSASIGGKSAFSIKSGTSMATPHVAGLAAYLIGLGGLSSPAAIASKIASIGTKGSVKDPKGSVNLIAYNGNGA |
| Enzyme Length | 400 |
| Uniprot Accession Number | L8G6I7 |
| Absorption | |
| Active Site | ACT_SITE 160; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 192; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 345; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Major secreted subtilisin-like serine endopeptidase (PubMed:25785714). Mediates the degradation of collagen, the major structural protein in the mammalian host. Degrades the nonhelical regions of collagen that function in the cross-linking of the helical components (By similarity). May function as virulence factor involved in epidermal wing necrosis observed in white nose syndrome (WNS) in bats (Probable). {ECO:0000250|UniProtKB:L8FSM5, ECO:0000269|PubMed:25785714, ECO:0000305|PubMed:25785714}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. {ECO:0000269|PubMed:25785714}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6 to 8. {ECO:0000269|PubMed:25785714}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (2); Glycosylation (1); Propeptide (1); Signal peptide (1); Site (1) |
| Keywords | Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25785714, ECO:0000269|PubMed:25944934}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,739 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |