| IED ID | IndEnz0002009518 |
| Enzyme Type ID | protease009518 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 36 EC 3.4.19.12 Deubiquitinating enzyme 36 Protein scrawny Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
| Gene Name | Usp36 scny GH16215 |
| Organism | Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Hawaiian Drosophila picture wing clade grimshawi clade grimshawi group grimshawi subgroup Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi) |
| Enzyme Sequence | MPVSLAVCETTANVVNAALRESLGSCVARAALSADEAKTSNGGGDGSSTSGSSTDNLQSQIVANAKRVLLAKIDYEEVDNYHESVLAKLKSKYIVIKPDSNGGAASNGNGNYNGSNKTNGKFGAGNGHDNNGNGNGIVNGGTSALNGGNNRKQIVVVDHQSSNQHGSPSNPNELPKPKRVLYQREHIRIGWKQSERKWQVGTGMLNVGNTCYLNSSLQALFHIPSLANWLVSESAHLENCNISESCGSNGCIICAMAKTLQSTQSNQSAVRPFLIYSKLRQICKHMVVGRQEDAHEFLRFLIEAMEKAYLMRFRNYKELDQLVKETTPLNQIFGGYLRSEVRCLSCNHVSITFQHFQDLLLDIRKSDTLEDAFDGYFSRERLEDMGYKCEGCKKKVSATKQFSLERAPITLCIQLKRFSMMGNKLTKQISFKPRIDLSRFAARSPTAAAQPLSYRLVSMVTHLGVSQHCGHYTAIGLTETGSYYNFDDSCVRPIAMQSVCNTNAYIMFYELDVNGNGHVVAPKMNGMRLTTNGGQQHSSPVVATAPAAVVSATATSTSASVSAAAVTSPRFIGPQLPNGYGNSNGHALGGGAAKTAIQFKTTPQKHQQQQQAQTQYQLNGHINGAAKFQTGAANANANKSSCNTLNNSKQHQPQQQQQQPQHILPISSDEEEDSDDDNDNDNVKTNKAPQLPSMPKMFEESSESVALTAKLKPKTALKSLVPYESASEEEEQQQQQQQQTLQQQAATNSRKRRSGADSSDTDDDDDEEQQQQQQQQPSLILRNGHAKTNGNLLNSSSSKTKSASNASSANVNSSKQKTDAIDEIFKSLNNYKNKHRNDHNHVDDDDDDDEDEDEDEDEAQAQAEKKTVTKSSSSSSSTSLTNGWQQSQNGKATASPKTPPSPAVIKTKTGIWQVTRNDEDDDENVDGVADADDDDDNDEVAEPAVVTAKNHKNPFAAGKATATTDANPSAKRQKLLNGSSKSQQTTPRIGNGYQGESLPNGNAVVSELLKQNHRGYGSSVLSWNGKASELDKETFDLVCAKRIAGYGAAAADEHCCDVNSGHSSNYGTNYKSLNNDMSSSSSSSSSTNSSSNSSSRSNGNSSNCPPCDLLAEAREQRKRDDDDEEENEMDRGRQRKIKSASVKCGSGATAAPPGYNPFQEYESQKRWHSNKSGSYSRFYHHPNYRSNFQQRNKFKHNRFAGGGGGAKFQQQRALQRHLASGGGFTRRQHHQSSGQQQQQS |
| Enzyme Length | 1240 |
| Uniprot Accession Number | B4IXE0 |
| Absorption | |
| Active Site | ACT_SITE 211; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 471; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Controls selective autophagy activation by ubiquitinated proteins. {ECO:0000250|UniProtKB:Q9VRP5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (11); Domain (1); Modified residue (6); Region (7) |
| Keywords | Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. |
| Modified Residue | MOD_RES 715; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 725; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 727; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 895; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 898; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 901; /note=Phosphoserine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 134,998 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |