| IED ID | IndEnz0002009534 |
| Enzyme Type ID | protease009534 |
| Protein Name |
Ribosomal large subunit pseudouridine synthase D EC 5.4.99.23 23S rRNA pseudouridine 1911/1915/1917 synthase rRNA pseudouridylate synthase D rRNA-uridine isomerase D |
| Gene Name | rluD sfhB yfiI b2594 JW2576 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQRVLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDLVVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVESLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHFRVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDRQALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL |
| Enzyme Length | 326 |
| Uniprot Accession Number | P33643 |
| Absorption | |
| Active Site | ACT_SITE 139 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=uridine(1911/1915/1917) in 23S rRNA = pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524, Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.23; Evidence={ECO:0000269|PubMed:11087118, ECO:0000269|PubMed:17937767}; |
| DNA Binding | |
| EC Number | 5.4.99.23 |
| Enzyme Function | FUNCTION: Responsible for synthesis of pseudouridine from uracil at positions 1911, 1915 and 1917 in 23S ribosomal RNA. Isomerization occurs as a late step during the assembly of the large ribosomal subunit. {ECO:0000269|PubMed:11087118, ECO:0000269|PubMed:17937767}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (15); Chain (1); Domain (1); Frameshift (1); Helix (10); Initiator methionine (1); Mutagenesis (1); Sequence conflict (1); Turn (2) |
| Keywords | 3D-structure;Direct protein sequencing;Isomerase;RNA-binding;Reference proteome;rRNA processing |
| Interact With | P21165; P0A8A4 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | Electron microscopy (1); X-ray crystallography (4) |
| Cross Reference PDB | 1PRZ; 1QYU; 1V9F; 2IST; 7BL5; |
| Mapped Pubmed ID | 14659742; 14730022; 15078091; 15690043; 16606699; 33639093; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,122 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:42524 |
| Cross Reference Brenda | 5.4.99.23; |