| IED ID | IndEnz0002009556 |
| Enzyme Type ID | protease009556 |
| Protein Name |
Serine protease SplE EC 3.4.21.- |
| Gene Name | splE SAOUHSC_01936 |
| Organism | Staphylococcus aureus (strain NCTC 8325 / PS 47) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus aureus Staphylococcus aureus (strain NCTC 8325 / PS 47) |
| Enzyme Sequence | MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYINKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK |
| Enzyme Length | 238 |
| Uniprot Accession Number | Q2FXC7 |
| Absorption | |
| Active Site | ACT_SITE 75; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 113; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 191; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (16); Chain (1); Helix (3); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal |
| Interact With | |
| Induction | INDUCTION: Positively regulated by agr (accessory gene regulator). {ECO:0000269|PubMed:11179322}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..36; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5MM8; |
| Mapped Pubmed ID | 29526434; |
| Motif | |
| Gene Encoded By | |
| Mass | 25,679 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |