IED Industry Enzyme Database

Detail Information for IndEnz0002009574
IED ID IndEnz0002009574
Enzyme Type ID protease009574
Protein Name Serine protease 1
EC 3.4.21.4
Anionic trypsin I
Anionic trypsin-I
Beta-trypsin
Cationic trypsin
Pretrypsinogen I
Trypsin I
Trypsin-I

Cleaved into: Alpha-trypsin chain 1; Alpha-trypsin chain 2
Gene Name PRSS1 TRP1 TRY1 TRYP1
Organism Bos taurus (Bovine)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine)
Enzyme Sequence MKTFIFLALLGAAVAFPVDDDDKIVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN
Enzyme Length 246
Uniprot Accession Number P00760
Absorption
Active Site ACT_SITE 63; /note=Charge relay system; ACT_SITE 107; /note=Charge relay system; ACT_SITE 200; /note=Charge relay system
Activity Regulation ACTIVITY REGULATION: Is inhibited by scorpion cyclotide trypsin inhibitor TopI1. {ECO:0000269|PubMed:32787139}.
Binding Site BINDING 200; /note=Substrate
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4;
DNA Binding
EC Number 3.4.21.4
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (17); Binding site (1); Chain (3); Disulfide bond (6); Domain (1); Helix (4); Metal binding (4); Propeptide (1); Region (2); Signal peptide (1); Turn (6)
Keywords 3D-structure;Calcium;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen
Interact With P00974; P01009
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space.
Modified Residue
Post Translational Modification PTM: Autocatalytic cleavage after Lys-23 leads to beta-trypsin by releasing a terminal hexapeptide. Subsequent cleavage after Lys-148 leads to alpha-trypsin. Further cleavage after Lys-193 yields pseudotrypsin. A cleavage may also occur after Arg-122.; PTM: Not sulfated on tyrosine residue(s).
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000269|PubMed:5967094
Structure 3D Neutron diffraction (7); Electron microscopy (1); X-ray crystallography (535)
Cross Reference PDB 1AQ7; 1AUJ; 1AZ8; 1BJU; 1BJV; 1BTP; 1BTW; 1BTX; 1BTY; 1BTZ; 1C1N; 1C1O; 1C1P; 1C1Q; 1C1R; 1C1S; 1C1T; 1C2D; 1C2E; 1C2F; 1C2G; 1C2H; 1C2I; 1C2J; 1C2K; 1C2L; 1C2M; 1C5P; 1C5Q; 1C5R; 1C5S; 1C5T; 1C5U; 1C5V; 1C9T; 1CE5; 1D6R; 1EB2; 1EJM; 1EZX; 1F0T; 1F0U; 1F2S; 1G36; 1G3B; 1G3C; 1G3D; 1G3E; 1G9I; 1GBT; 1GHZ; 1GI0; 1GI1; 1GI2; 1GI3; 1GI4; 1GI5; 1GI6; 1GJ6; 1HJ9; 1J8A; 1JIR; 1JRS; 1JRT; 1K1I; 1K1J; 1K1L; 1K1M; 1K1N; 1K1O; 1K1P; 1LQE; 1MAX; 1MAY; 1MTS; 1MTU; 1MTV; 1MTW; 1N6X; 1N6Y; 1NC6; 1NTP; 1O2H; 1O2I; 1O2J; 1O2K; 1O2L; 1O2M; 1O2N; 1O2O; 1O2P; 1O2Q; 1O2R; 1O2S; 1O2T; 1O2U; 1O2V; 1O2W; 1O2X; 1O2Y; 1O2Z; 1O30; 1O31; 1O32; 1O33; 1O34; 1O35; 1O36; 1O37; 1O38; 1O39; 1O3A; 1O3B; 1O3C; 1O3D; 1O3E; 1O3F; 1O3G; 1O3H; 1O3I; 1O3J; 1O3K; 1O3L; 1O3M; 1O3N; 1O3O; 1OPH; 1OX1; 1OYQ; 1P2I; 1P2J; 1P2K; 1PPC; 1PPE; 1PPH; 1QA0; 1QB1; 1QB6; 1QB9; 1QBN; 1QBO; 1QCP; 1QL7; 1QL8; 1RXP; 1S0Q; 1S0R; 1SBW; 1SFI; 1SMF; 1TAB; 1TAW; 1TGB; 1TGC; 1TGN; 1TGS; 1TGT; 1TIO; 1TLD; 1TNG; 1TNH; 1TNI; 1TNJ; 1TNK; 1TNL; 1TPA; 1TPO; 1TPP; 1TPS; 1TX7; 1TX8; 1TYN; 1UTN; 1UTO; 1UTP; 1UTQ; 1V2J; 1V2K; 1V2L; 1V2M; 1V2N; 1V2O; 1V2P; 1V2Q; 1V2R; 1V2S; 1V2T; 1V2U; 1V2V; 1V2W; 1XUF; 1XUG; 1XUH; 1XUI; 1XUJ; 1XUK; 1Y3U; 1Y3V; 1Y3W; 1Y3X; 1Y3Y; 1Y59; 1Y5A; 1Y5B; 1Y5U; 1YP9; 1YYY; 1ZR0; 1ZZZ; 2A7H; 2AGE; 2AGG; 2AGI; 2AH4; 2AYW; 2BLV; 2BLW; 2BTC; 2BY5; 2BY6; 2BY7; 2BY8; 2BY9; 2BYA; 2BZA; 2CMY; 2D8W; 2F3C; 2FI3; 2FI4; 2FI5; 2FTL; 2FTM; 2FX4; 2FX6; 2G55; 2G5N; 2G5V; 2G81; 2G8T; 2ILN; 2J9N; 2O9Q; 2OTV; 2OXS; 2PLX; 2PTC; 2PTN; 2QN5; 2QYI; 2TGA; 2TGD; 2TGP; 2TGT; 2TIO; 2TLD; 2TPI; 2UUY; 2XTT; 2ZDK; 2ZDL; 2ZDM; 2ZDN; 2ZFS; 2ZFT; 2ZHD; 2ZQ1; 2ZQ2; 3A7T; 3A7V; 3A7W; 3A7X; 3A7Y; 3A7Z; 3A80; 3A81; 3A82; 3A83; 3A84; 3A85; 3A86; 3A87; 3A88; 3A89; 3A8A; 3A8B; 3A8C; 3A8D; 3AAS; 3AAU; 3AAV; 3ATI; 3ATK; 3ATL; 3ATM; 3BTD; 3BTE; 3BTF; 3BTG; 3BTH; 3BTK; 3BTM; 3BTQ; 3BTT; 3BTW; 3D65; 3E8L; 3GY2; 3GY3; 3GY4; 3GY5; 3GY6; 3GY7; 3GY8; 3I29; 3ITI; 3LJJ; 3LJO; 3M35; 3M7Q; 3MFJ; 3MI4; 3NK8; 3NKK; 3OTJ; 3PLB; 3PLK; 3PLP; 3PM3; 3PMJ; 3PTB; 3PTN; 3PWB; 3PWC; 3PYH; 3Q00; 3QK1; 3RDZ; 3RU4; 3RXA; 3RXB; 3RXC; 3RXD; 3RXE; 3RXF; 3RXG; 3RXH; 3RXI; 3RXJ; 3RXK; 3RXL; 3RXM; 3RXO; 3RXP; 3RXQ; 3RXR; 3RXS; 3RXT; 3RXU; 3RXV; 3T25; 3T26; 3T27; 3T28; 3T29; 3TPI; 3UNQ; 3UNR; 3UNS; 3UOP; 3UPE; 3UQO; 3UQV; 3UUZ; 3UWI; 3UY9; 3V0X; 3V12; 3V13; 3VEQ; 3VPK; 4AB8; 4AB9; 4ABA; 4ABB; 4ABD; 4ABE; 4ABF; 4ABG; 4ABH; 4ABI; 4ABJ; 4AOQ; 4AOR; 4B1T; 4B2A; 4B2B; 4B2C; 4GUX; 4HGC; 4I8G; 4I8H; 4I8J; 4I8K; 4I8L; 4J2Y; 4KTS; 4KTU; 4MTB; 4NCY; 4NIV; 4NIW; 4NIX; 4NIY; 4TPI; 4TPY; 4U2W; 4XOJ; 4Y0Y; 4Y0Z; 4Y10; 4Y11; 4YTA; 5EG4; 5F6M; 5FXL; 5GIB; 5GXP; 5JYI; 5K7R; 5LGO; 5LH4; 5LH8; 5MN1; 5MNA; 5MNB; 5MNC; 5MNE; 5MNF; 5MNG; 5MNH; 5MNK; 5MNL; 5MNM; 5MNN; 5MNO; 5MNP; 5MNQ; 5MNX; 5MNY; 5MNZ; 5MO0; 5MO1; 5MO2; 5MON; 5MOO; 5MOP; 5MOQ; 5MOR; 5MOS; 5PTP; 5T3H; 6AVL; 6B6N; 6B6O; 6B6P; 6B6Q; 6B6R; 6B6S; 6B6T; 6BFP; 6BVH; 6DWF; 6DWH; 6DWR; 6DWU; 6DZF; 6E5M; 6EAT; 6EAU; 6EAV; 6EAW; 6EAX; 6FID; 6MRQ; 6QIH; 6QL0; 6SUX; 6SV0; 6SV6; 6SV8; 6SV9; 6SVB; 6SVD; 6SVG; 6SVI; 6SVJ; 6SVN; 6SVR; 6SVU; 6SVV; 6SVW; 6SVX; 6SVZ; 6SW0; 6SWV; 6SY3; 6T0M; 6T0P; 6T5W; 6T9U; 6T9V; 6U22; 6VXY; 6XYG; 6XYK; 6YDY; 6YIS; 6YIT; 6YIU; 6YIV; 6YIW; 6YIX; 6YIY; 6YZA; 6YZC; 6ZFJ; 6ZFK; 6ZQ2; 7AL8; 7AYS; 7BRV; 7BRW; 7BRX; 7BRY; 7BRZ; 7BS0; 7BS1; 7BS2; 7BS3; 7BS4; 7BS5; 7BS6; 7BS7; 7BS8; 7BS9; 7BSA; 7JR1; 7JR2; 7JWX;
Mapped Pubmed ID 10089404; 10222201; 10390350; 10417407; 10447205; 10497030; 10512718; 10524768; 10531473; 10651279; 10772864; 10779411; 10926503; 10966741; 11152605; 11257512; 11264577; 11292354; 11342132; 11676542; 11731301; 11738605; 11881991; 11921406; 12061878; 12575941; 12742021; 12860985; 12930148; 14568540; 14640539; 14729347; 15044735; 15081015; 15380220; 1557349; 1584773; 15932872; 16081330; 16131756; 16131760; 16321984; 16374786; 16421442; 16510975; 16636277; 16681368; 16754971; 17142058; 17142290; 17157870; 17191291; 17327674; 17372355; 17391695; 18084102; 18692070; 1879520; 19407425; 19461582; 19461845; 19640842; 19822883; 19851024; 20202854; 20356563; 20886466; 21097875; 21111747; 21245536; 21387509; 21698291; 21707922; 22115549; 22187139; 22194335; 2226434; 22374650; 2252895; 22683792; 22709512; 22751665; 22975140; 23161653; 23275169; 23626794; 23897468; 24034223; 24169696; 24520050; 24591244; 24598736; 24798798; 24816088; 25003390; 25003391; 25546528; 26027487; 2614845; 26212347; 27035972; 27280436; 27476144; 27487827; 27841370; 28192420; 28371253; 2914611; 29449928; 29979188; 30177695; 30198901; 3032921; 30644845; 30668585; 30817908; 30876891; 31848402; 32011145; 32270580; 32381789; 33399553; 34094189; 34342281; 6207021; 668890; 6712567; 7169635; 7498454; 7548040; 7599119; 7634078; 7798176; 8605148; 864704; 8845765; 8939944; 9300481; 9341205; 9383379; 9468142; 9724521; 9836602; 9876114; 9920392;
Motif
Gene Encoded By
Mass 25,785
Kinetics
Metal Binding METAL 75; /note=Calcium; METAL 77; /note=Calcium; via carbonyl oxygen; METAL 80; /note=Calcium; via carbonyl oxygen; METAL 85; /note=Calcium
Rhea ID
Cross Reference Brenda 3.4.21.4;