| IED ID | IndEnz0002009585 |
| Enzyme Type ID | protease009585 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 15 EC 3.4.19.12 Deubiquitinating enzyme 15 Ubiquitin thioesterase 15 Ubiquitin-specific-processing protease 15 |
| Gene Name | usp15 |
| Organism | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Silurana Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Enzyme Sequence | MAEGGVVDLETQRSEVSALLKTPLRRGDTWYLIDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDTDTQSLKEHLIDELDYILLPTEGWNKLVSWYSVVESQQPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDMIEKEMRTIFSIPDEKETRLWNKYMSNTFEPLNKPESTIQDAGLYQGQMLVIEQKNQEGQWPRGSMPKSSGIPNFSTLPKISPSSLSNNYNNFNSRIMKNSNYCLPSYAAYKNYEYSEPGRHNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQDELNMDNPLGMRGEIAKSYAELIKQMWSGKYSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHIKRNDSIIVDIFHGLFKSTLVCPECSKISVTFDPFCYLTLPLPMKKERALEVYLVRMDPLAKPMQYKVIVPKIGNIMDLCTALSSLSGIAPEKMVVTDIYNHRFHRIFAMDENLSSIMERDDIYVFETSINRTEDTEQVIIPVYLREKFRHTSYSHHHGSTLFGQPFLITVPRNITEDKLYNLLLLRMCRYVKATNDTEENDGSLHCNKEHTVNGNGPNGIHEEGSPSEMETDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEDTCKGQSVTGQKKRLFTFQFSNLGSSDITYIKDDTKYIRFDERQLRLDERSYLALDWDPKLKKKFFDENAAEDFEKHESVDFTPQKKAFMKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPISDLDMSTFLINPNAGPCCYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEEQIVSKAAYVLFYQRQDTITGTGFFPLDKEVKQGASAATGAPHESDEESNEDENDIENENCMHTN |
| Enzyme Length | 982 |
| Uniprot Accession Number | F6Z5C0 |
| Absorption | |
| Active Site | ACT_SITE 297; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 891; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q9Y4E8}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs, thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of tgfbr1, leading to an enhanced TGF-beta signal. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B. Involved in endosome organization by mediating deubiquitination of rnf26 target(s), releasing vesicles that are restrained in the perinuclear region. {ECO:0000250|UniProtKB:Q9Y4E8}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Compositional bias (2); Domain (2); Region (2); Sequence conflict (2) |
| Keywords | Alternative splicing;Cytoplasm;Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Y4E8}. Nucleus {ECO:0000250|UniProtKB:Q9Y4E8}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 112,689 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |