| IED ID | IndEnz0002009591 |
| Enzyme Type ID | protease009591 |
| Protein Name |
Trypsin EC 3.4.21.4 |
| Gene Name | |
| Organism | Sarcophaga bullata (Grey flesh fly) (Neobellieria bullata) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Calyptratae Oestroidea Sarcophagidae (flesh flies) Sarcophaginae Sarcophaga Neobellieria Sarcophaga bullata (Grey flesh fly) (Neobellieria bullata) |
| Enzyme Sequence | MLRFIAVFALVNCALAGTLPNDLDGRIVNGVDTTIEAHPYQVPLQNAALSHFCGGSIISEDLVVTAAHCMQSYTASQIKVRLGSTIYNEGGELVSVKAFKFHEGYNPKTMVNDVALIKLATPVRESSKIRYIRLADRTPPTGTPAVVTGWGTKCFLTCVSLPKTLQEVEVDIVDQKACASNEFKYGSQIQDTMVCAYALKKDACQGDSGGPLVANNQLVGIVSWGSGCARVGYPGVFCDVPSVRSWIEKTAKEL |
| Enzyme Length | 254 |
| Uniprot Accession Number | P51588 |
| Absorption | |
| Active Site | ACT_SITE 68; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 113; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 208; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | FUNCTION: Involved in digestion of a protein meal. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Domain (1); Propeptide (1); Signal peptide (1); Site (1) |
| Keywords | Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,309 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |