| IED ID | IndEnz0002009652 |
| Enzyme Type ID | protease009652 |
| Protein Name |
Ubiquitin thioesterase trabid dTrbd EC 3.4.19.12 |
| Gene Name | trbd CG9448 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MCDTKDDAQKWKCETCTYENYPSSLKCTMCQASKPLLNEDIFRLSPAQESCTVAEEAAAVEVAVMSPTPSSTCYSLQPQSQARQSNVADSEKWPCKVCTYLNWPRSLRCVQCCTKRGGEAIERGKKDMDNEADGDRAGEALQALRISGSEENLANKPVQLIGATASHRLSLSRGIDDATHLNNLANASHNQSQSQHRQPVLQQQMQLQLQPQQQRESSSSAAVPPQQQKQCYVSKWACNSCTYENWPRSIKCSMCGKTREREISGSQNDLHASSSLNSQEENQQQLQQPNVDTVSVNNSFNKKHIYQLGSSETINNCDTLQERQERRQRQIRRQVDWQWLNACLGVVENNYSAVEAYLSCGGNPARSLTSTEIAALNRNSAFDVGHTLIHLAIRFHREEMLPMLLDQISGSGPGIKRVPSYVAPDLAADIRRHFANTLRLRKSGLPCHYVQKHATFALPAEIEELPIPIQEQLYDELLDRDAQKQLETPPPALNWSLEITARLSSRMFVLWNRSAGDCLLDSAMQATWGVFDRDNILRRALADTLHQCGHVFFTRWKEYEMLQASMLHFTLEDSQFEEDWSTLLSLAGQPGSSLEQLHIFALAHILRRPIIVYGVKYVKSFRGEDIGYARFEGVYLPLFWDQNFCTKSPIALGYTRGHFSALVPMEPFTRIDGRRDDVEDVTYLPLMDCELKLLPIHFLTQSEVGNEESMMRQWLDVCVTDGGLLVAQQKLSKRPLLVAQMLEEWLNHYRRIAQVITAPFIRRPQITHYSSDGDSDEE |
| Enzyme Length | 778 |
| Uniprot Accession Number | Q9VH90 |
| Absorption | |
| Active Site | ACT_SITE 518; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 658; /note=Proton acceptor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Positive regulator of the Wnt signaling pathway. Specifically cleaves 'Lys-63'-linked ubiquitin chains. May act by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription (By similarity). Required for an efficient wg response, but not for other signaling responses, in the eye. {ECO:0000250, ECO:0000269|PubMed:18281465}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Erroneous initiation (1); Modified residue (3); Region (2); Zinc finger (3) |
| Keywords | Hydrolase;Metal-binding;Phosphoprotein;Protease;Reference proteome;Repeat;Thiol protease;Ubl conjugation pathway;Wnt signaling pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 770; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 771; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897; MOD_RES 775; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18327897 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10664582; 11102367; 14684833; 16369566; 19154620; 20220848; 20371351; 21074052; 21834987; 22253573; 22937016; 23071443; 23139745; 23275879; 23899565; 24362437; 24586180; 25687947; 25954029; 26551273; 26588485; 27172210; 28413160; 28445733; 29084807; 29146454; 31794428; 32774336; 33092519; 33988679; |
| Motif | |
| Gene Encoded By | |
| Mass | 88,199 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |