| IED ID | IndEnz0002009657 |
| Enzyme Type ID | protease009657 |
| Protein Name |
Ubiquitin C-terminal hydrolase 12 EC 3.4.19.12 Deubiquitinating enzyme 12 AtUBP12 Ubiquitin thioesterase 12 Ubiquitin-specific-processing protease 12 |
| Gene Name | UBP12 At5g06600 F15M7.13 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MTMMTPPPVDQPEDEEMLVPNSDLVDGPAQPMEVTQPETAASTVENQPAEDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKGNNVDHLSMYLDVSDAASLPYGWSRYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSELYDPSRGYLVNDTVLVEAEVAVRKVLDYWSYDSKKETGFVGLKNQGATCYMNSLLQTLYHIPYFRKAVYHMPTTENDAPTASIPLALQSLFYKLQYNDTSVATKELTKSFGWDTYDSFMQHDVQELNRVLCEKLEDKMKGTVVEGTIQQLFEGHHMNYIECINVDFKSTRKESFYDLQLDVKGCKDVYASFDKYVEVERLEGDNKYHAEGHGLQDAKKGVLFIDFPPVLQLQLKRFEYDFMRDTMVKINDRYEFPLELDLDREDGKYLSPDADRSVRNLYTLHSVLVHSGGVHGGHYYAFIRPTLSDQWYKFDDERVTKEDLKRALEEQYGGEEELPQTNPGFNNNPPFKFTKYSNAYMLVYIRESDKDKIICNVDEKDIAEHLRVRLKKEQEEKEDKRRYKAQAHLYTIIKVARDEDLKEQIGKDIYFDLVDHDKVRSFRIQKQTPFQQFKEEVAKEFGVPVQLQRFWIWAKRQNHTYRPNRPLTPQEELQPVGQIREASNKANTAELKLFLEVEHLDLRPIPPPEKSKEDILLFFKLYDPEKAVLSYAGRLMVKSSSKPMDITGKLNEMVGFAPDEEIELFEEIKFEPCVMCEHLDKKTSFRLCQIEDGDIICFQKPLVNKEIECLYPAVPSFLEYVQNRQLVRFRALEKPKEDEFVLELSKQHTYDDVVEKVAEKLGLDDPSKLRLTSHNCYSQQPKPQPIKYRGVDHLSDMLVHYNQTSDILYYEVLDIPLPELQGLKTLKVAFHHATKEEVVIHNIRLPKQSTVGDVINELKTKVELSHPDAELRLLEVFYHKIYKIFPSTERIENINDQYWTLRAEEIPEEEKNIGPNDRLILVYHFAKETGQNQQVQNFGEPFFLVIHEGETLEEIKNRIQKKLHVSDEDFAKWKFAFMSMGRPEYLQDTDVVYNRFQRRDVYGAFEQYLGLEHADTTPKRAYAANQNRHAYEKPVKIYN |
| Enzyme Length | 1116 |
| Uniprot Accession Number | Q9FPT1 |
| Absorption | |
| Active Site | ACT_SITE 208; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01035, ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 455; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01035, ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity). Positive regulator of root meristem development that, together with UBP13, prevents the ubiquitination and turnover of RGFR1 induced by the RGF1 hormone peptide, thus influencing PLT1 and PLT2 expression (PubMed:29339500). {ECO:0000250|UniProtKB:Q9FPT5, ECO:0000269|PubMed:29339500}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Compositional bias (1); Domain (2); Erroneous gene model prediction (1); Region (1); Sequence conflict (1) |
| Keywords | Alternative splicing;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15010611; 15247402; 16166256; 16897085; 18485060; 18775970; 21388379; 23645632; 27525512; 28536144; 28627464; 28695315; 30833711; 31050353; 31105721; 32209225; 32546254; 34732572; |
| Motif | |
| Gene Encoded By | |
| Mass | 130,607 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |