| IED ID | IndEnz0002009664 |
| Enzyme Type ID | protease009664 |
| Protein Name |
Ubl carboxyl-terminal hydrolase 18 EC 3.4.19.- 43 kDa ISG15-specific protease ISG15-specific-processing protease Ubl thioesterase 18 |
| Gene Name | Usp18 Ubp43 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MGKGFGLLRKPCQSVVAEPQQYSALEEERTMKRKRVLSRDLCSAWDSPHGLVGLHNIGQTCCLNSLLQVFMMNMDFRMILKRITVPRSAEERKRSVPFQLLLLLEKMQDSRQKAVLPTELVQCLQKYNVPLFVQHDAAQLYLTIWNLTKDQITDTDLTERLQGLFTIWTQESLICVGCTAESSRRSKLLTLSLPLFDKDAKPLKTLEDALRCFVQPKELASSDMCCESCGEKTPWKQVLKLTHLPQTLTIHLMRFSARNSRTEKICHSVNFPQSLDFSQVLPTEEDLGDTKEQSEIHYELFAVIAHVGMADFGHYCAYIRNPVDGKWFCFNDSHVCWVTWKDVQCTYGNHRYRWRETAYLLVYTKTGS |
| Enzyme Length | 368 |
| Uniprot Accession Number | Q9WTV6 |
| Absorption | |
| Active Site | ACT_SITE 61; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 314; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.19.- |
| Enzyme Function | FUNCTION: Involved in the negative regulation of the inflammatory response triggered by type I interferon. Upon recruitment by STAT2 to the type I IFN receptor subunit IFNAR2 interferes with the assembly of the ternary interferon-IFNAR1-IFNAR2 complex and acts as a negative regulator of the type I IFN signaling pathway. Also regulates protein ISGylation. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. {ECO:0000250|UniProtKB:Q9UMW8}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (17); Chain (1); Domain (1); Helix (11); Region (4); Sequence conflict (2); Turn (2) |
| Keywords | 3D-structure;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | Q64339 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 5CHT; 5CHV; |
| Mapped Pubmed ID | 10777664; 11217851; 11788588; 12208842; 12466851; 12600939; 15342634; 15531891; 16002682; 16139798; 16314524; 16382139; 16602821; 16710296; 17349616; 17381847; 18802094; 18941237; 19551150; 19564419; 20693420; 21267068; 21614019; 21677750; 22491252; 22683641; 23012479; 23291318; 23681607; 23825189; 24204252; 24533902; 24648452; 24807690; 24884733; 25165091; 25605921; 25805508; 25896511; 26553386; 26555296; 27009955; 27572150; 27721430; 27882925; 28165509; 28165510; 28242811; 28557172; 28718215; 30266866; 31272490; 31513843; 31772204; 31974171; 32195343; 32686110; 32726803; 33380466; 33694203; 34016972; |
| Motif | |
| Gene Encoded By | |
| Mass | 42,472 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |