Detail Information for IndEnz0002009669
IED ID IndEnz0002009669
Enzyme Type ID protease009669
Protein Name Abasic site processing protein YobE
EC 3.4.-.-
Gene Name yobE BSU18880
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MCGKFTLFSEFDDIIEQFNIDQFLPEGEYHPSYNVAPSQNILTIINDGSNNRLGKLRWGLIPPCAKDEKIGYKMINARAETLAEKPSFRKPLGSKRCIIPADSFYEWKRLDPKTKIPMRIKLKSSNLFAFAGLYEKWNTLEGNLLYTCTIITIKPSELMEDIHDRMPVILTDENKKEWLNPKNTDPDYLQSLLLPYDADDMEAYQVSSLVNSPELIESH
Enzyme Length 219
Uniprot Accession Number O34915
Absorption
Active Site ACT_SITE 2; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q96FZ2
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (By similarity). May act as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity). {ECO:0000250|UniProtKB:P76318, ECO:0000250|UniProtKB:Q8R1M0}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Initiator methionine (1); Modified residue (1); Site (2)
Keywords Covalent protein-DNA linkage;DNA damage;DNA-binding;Hydrolase;Protease;Reference proteome;SOS response
Interact With
Induction
Subcellular Location
Modified Residue MOD_RES 2; /note=Thiazolidine linkage to a ring-opened DNA abasic site; /evidence=ECO:0000250|UniProtKB:P76318
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 25,241
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda