| IED ID | IndEnz0002009675 |
| Enzyme Type ID | protease009675 |
| Protein Name |
ATP-dependent DNA helicase PIF2 EC 3.6.4.12 DNA repair and recombination helicase PIF2 |
| Gene Name | PIF2 Tb11.02.4740 |
| Organism | Trypanosoma brucei brucei (strain 927/4 GUTat10.1) |
| Taxonomic Lineage | cellular organisms Eukaryota Discoba Euglenozoa Kinetoplastea (kinetoplasts) Metakinetoplastina Trypanosomatida Trypanosomatidae Trypanosoma Trypanozoon Trypanosoma brucei Trypanosoma brucei brucei Trypanosoma brucei brucei (strain 927/4 GUTat10.1) |
| Enzyme Sequence | MSSKTVVWSSLGRFMRTSHHRLLDEKLFPASHNRHVQSRLLSTPLSLIAVPSWMSKCRTSAVRLSRHPPQVSGQEPCAVTASSSVGTWHGSSPAGLRRCQHVRSLNGWTTPGDVPIRHGSRSIATIATGCGGVGAGGINSSVDVAEVSGVSTSGVAAAPTVALPPGPPVEKPPLICVVRRATRGGAAADSVGTGTPGKCDKPPDSSNTLVGTIARVLSSSGECGRHSNANQEGYSLGSRPARKILVKLEADTVAAPLADTKEKQIYVQQQPVVVAQSPLARSSSTVTWEEGDTLVYNIFTQRVVKSSSASIRALSVLGVGVRSSEKELSVVDPHALVEFRDKLREKEVSWPSAWRSSLFNQLQHVLLKDQPMEEMISRVHELLQLHYQRVKRSCVANVTKGEPDTKTEEANPAGDEVAINGEMTYHERLLGYPDLNEEQRRVVDFVLRGYNTYIGGGAGTGKSLLLRVIRQELVSRGLTVATTATTGIAARRLNGATLHHCFGVNVYGEFTRRAELKEFDVIIIDEVSMLSKELFESLEFQLRRANGVDLPFGGVQVILSGDFLQLGAICSVSLVHSSVFRRNFAMLKLQRVVRQEGSSIFAQQLQELRRGTVPHDLQDTVQFLSPPETAKWLEGEGKGAVKLLPTNKEVDEVNQAELDKLPSDLVVYPAQMQAPSLVGRWTATYILEAVVKDTKMIDTHKLTRALEQYVLDFLQKTPYASDYTLPVVGQRYIVLYKLFVDAFAFRVRIPQDMSEKDMRDLALHLRGLETWLPACGLGVFLREILDSPDGLHTDADDYTLTRYAELHPMASPLRLKKGAKVMLRTNLAPGLVNGSLGVVVGFKELSAKHLPRFVNTPGRIAAVENYAEYLRYEHGFTTAFAPEVDFGGGRVIVVPPVLFSVGGLSNTNHYHVGIVSLPLSLAYAFTVHKVQGLTLAGRVHLELSRMWPCDHLLYVAMSRVRNPEQLTVSSFHNSLVRCASECLLFDDSLPPVEQVRVLPHFFQASWQRTPSRRKAALQRKREQAKQSKQKKAAKLKEAMIKQAKEATP |
| Enzyme Length | 1048 |
| Uniprot Accession Number | Q384Y0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; Evidence={ECO:0000269|PubMed:19646907}; |
| DNA Binding | DNA_BIND 951..971; /evidence=ECO:0000255 |
| EC Number | 3.6.4.12 |
| Enzyme Function | FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of mitochondrial (kinetoplast) genome stability. Essential for replication of kinetoplast maxicircles, but not minicircles. {ECO:0000269|PubMed:19646907}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 456..463; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Compositional bias (1); DNA binding (1); Mutagenesis (1); Nucleotide binding (1); Region (2); Transit peptide (1) |
| Keywords | ATP-binding;DNA damage;DNA recombination;DNA repair;DNA-binding;Helicase;Hydrolase;Mitochondrion;Nucleotide-binding;Reference proteome;Transit peptide |
| Interact With | |
| Induction | INDUCTION: Protein level is regulated by mitochondrial proteasome-like protease HslVU. |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:19646907}. Note=Localizes throughout the tubular structure of the single mitochondrion. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 115,146 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:13065 |
| Cross Reference Brenda |