| IED ID | IndEnz0002009676 |
| Enzyme Type ID | protease009676 |
| Protein Name |
Polyprotein P3 Includes: Putative movement protein MP ; Capsid protein Coat protein CP ; Protease PR EC 3.4.23.- ; Reverse transcriptase RT EC 2.7.7.49 ; Ribonuclease H EC 3.1.26.4 |
| Gene Name | |
| Organism | Commelina yellow mottle virus (CoYMV) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Caulimoviridae Badnavirus Commelina yellow mottle virus (CoYMV) |
| Enzyme Sequence | MATRRLPAVTQTDGSRTATESGVPEYEDQIRSYRNDQRRRHIWAGRGRRLLSIMPGVSSSERTLEMQMNPEVQLQRSMNHRAEAVPAEVLYRTFHGSVNHRVYSHRSEERMMVVNGSQVDRSFIQESSFEVLSRTGIEFIHIGVMLVRIQILHRKFAGTMALIVFRDTRWSDDRAVLAAMEIDLSEGNQIVYVLPDIMMTIKSFYRHIQICVMTKGYDGWQGEDNLLITRGLTGRLSNTSNVGFAYDVKAMVEHLQSNGVKAIKGEKWDAKRFHNGQWNIEPSKVVVPMQPTEMKAVSNYDGTTSLRFSNYAAASTSKPPQYNEKDEEINEDEQEINHSLNLILNDEESTDEDEEYYQYQRYAWSQVGDSTFYYDTDGVWEEIDRCNDLPEYVPSETSTPTIDESEAIIDEFLEHAYEQRCDSDESLQSGDPRKYEYPTPQSSPEHLDNESRSRSSSASSTSMQDDVEEIVRLMKEMRMKKQKKKKAQQALSSQAQEEPIIEENIEENKQAQEEPTQEEIPTHKENQPEEIQNEEIHVFEEEPAFKHLAAQLSELVNMAESSGQSGVGFQPPVNAQPDVNMEGPAGYAPATSQATWSNGVNIPVKSANFRWKGPVGNFQLPSAQGKDGAMLVFGMNYSPEVFDRWASITRNYISSFNFNDGGDKIAWMEDLLGETERKIFVSWRMRFKDEYQNLAKIANQDGGTQAILSQIRRIFLGEDPVLGQNTVQNIAFRKLKQLVCPNYQSIRRYLMDYLTLAAETGLMWSETEGPAISEELFTKMPAAIGERVAQAYKIMDPTSAVNLPSRVYFTINYLTEQCKEASYMRSLKALDFCRDFPIEGYYGRSGEKKKYTARKATKYTGKAHDNHIRVTKAKYQRKCKCYICGQEGHYANQCRNKHKDQQRVAILQSLDLKENEEVVSADDKEEEDDEIFSVLGEEDYQEETIMVLEEDDIQQIIKEFSKFGDLSRRNVGPNFPGPAEVQMGVLKPKSSWRRPIQATLEEINCHHNWTAISTGQLACRSCKQFLAGVQCHHCHAVYCFMCAEAYHDVQAEKILSKDYSFSARGKKGKAVIIEEDEIEGEFLISQLQQENQRLQKQVERLQEELMKLHREKDEALKHSEKASRVFSTIQESDEAELNLIKEELRQFKEETRMAIAQLKEAIIVQEEDTIEERCAMILEEKHTENIYSATAKAEYNGLYNVKVGIKPDNMEPYYINAIVDTGATACLIQISAIPENYYEDAKVTVNFRSVLGIGTSTQMIKAGRILIGEQYFRMPVTYVMNMGLSPGIQMIIGCSFIRSLEGGLRIEKDIITFYKLVTSIETSRTTQVANSIEELELSEDEYLNIAASVETPSFLDQEFARKNKDLLKEMKEMKYIGENPMEFWKNNKIKCKLNIINPDIKIMGRPIKHVTPGDEEAMTRQINLLLQMKVIRPSESKHRSTAFIVRSGTEIDPITGKEKKGKERMVFNYKLLNENTESDQYSLPGINTIISKVGRSKIYSKFDLKSGFWQVAMEEESVPWTAFLAGNKLYEWLVMPFGLKNAPAIFQRKMDNVFKGTEKFIAVYIDDILVFSETAEQHSQHLYTMLQLCKENGLILSPTKMKIGTPEIDFLGASLGCTKIKLQPHIISKICDFSDEKLATPEGMRSWLGILSYARNYIQDIGKLVQPLRQKMAPTGDKRMNPETWKMVRQIKEKVKNLPDLQLPPKDSFIIIETDGCMTGWGAVCKWKMSKHDPRSTERICAYASGSFNPIKSTIDAEIQAAIHGLDKFKIYYLDKKELIIRSDCEAIIKFYNKTNENKPSRVRWLTFSDFLTGLGITVTFEHIDGKHNGLADALSRMINFIVEKNDESPYRFTSSVEDALKVCNDDHGRNLISAVINDIITVLRR |
| Enzyme Length | 1886 |
| Uniprot Accession Number | P19199 |
| Absorption | |
| Active Site | ACT_SITE 1220; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4; Evidence={ECO:0000255|PROSITE-ProRule:PRU00408}; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; |
| DNA Binding | |
| EC Number | 3.4.23.-; 2.7.7.49; 3.1.26.4 |
| Enzyme Function | FUNCTION: Capsid protein self assembles to form a bacilliform capsid about 90-900 nm in length. The capsid encapsulates the genomic dsDNA. Following virus entry into host cell, provides nuclear import of the viral genome. Virus particles do not enter the nucleus, but are targeted to the nuclear membrane through the interaction with host importins (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (2); Domain (3); Metal binding (5); Region (3); Zinc finger (1) |
| Keywords | Aspartyl protease;Endonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleotidyltransferase;Protease;RNA-directed DNA polymerase;Reference proteome;Transferase;Transport;Viral movement protein;Viral penetration into host nucleus;Virus entry into host cell;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Polyprotein P3 is presumably proteolytically cleaved into several chains by viral protease. {ECO:0000305}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 215,662 |
| Kinetics | |
| Metal Binding | METAL 1715; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 1715; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 1758; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 1784; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 1833; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408 |
| Rhea ID | RHEA:22508 |
| Cross Reference Brenda |