| IED ID | IndEnz0002009712 |
| Enzyme Type ID | protease009712 |
| Protein Name |
Serine protease SepA autotransporter EC 3.4.21.- Cleaved into: Serine protease SepA; Serine protease SepA translocator |
| Gene Name | sepA CP0070 |
| Organism | Shigella flexneri |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Shigella Shigella flexneri |
| Enzyme Sequence | MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVAALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAPMADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSGLDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAANGTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRVSALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDGTVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNLTFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYDSSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQLNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSDWETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVFSVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRVGSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTEGINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAVLRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNTTFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAGTQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNNLNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGALLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLGTKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKDYNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDSRMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF |
| Enzyme Length | 1364 |
| Uniprot Accession Number | Q8VSL2 |
| Absorption | |
| Active Site | ACT_SITE 134; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 162; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028; ACT_SITE 267; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01028 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the serine protease inhibitor PMSF, but not by benzamidine, alpha 1-antitrypsin, alpha 1-antichymotrypsin. Not inhibited by metalloprotease inhibitors such as EDTA and orthophenanthroline. {ECO:0000269|PubMed:9695914}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Major protein secreted in laboratory media showing proteolytic activity. May be involved in invasion and destruction of host intestinal epithelium. {ECO:0000269|PubMed:9695914}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 37 degrees Celsius.; |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (90); Chain (3); Domain (2); Helix (9); Natural variant (2); Sequence conflict (4); Signal peptide (1); Site (1); Turn (6) |
| Keywords | 3D-structure;Cell outer membrane;Direct protein sequencing;Hydrolase;Membrane;Periplasm;Plasmid;Protease;Reference proteome;Secreted;Serine protease;Signal;Transmembrane;Transmembrane beta strand;Virulence;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Serine protease SepA autotransporter]: Periplasm {ECO:0000250}.; SUBCELLULAR LOCATION: [Serine protease SepA]: Secreted. Cell surface.; SUBCELLULAR LOCATION: [Serine protease SepA translocator]: Cell outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved to release the mature protein from the outer membrane. Cleavage is performed by an unknown protease. |
| Signal Peptide | SIGNAL 1..56; /evidence=ECO:0000269|PubMed:7476198 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5J44; |
| Mapped Pubmed ID | 28598765; |
| Motif | |
| Gene Encoded By | Plasmid pWR100; Plasmid pCP301 |
| Mass | 146,017 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |