| IED ID | IndEnz0002009791 |
| Enzyme Type ID | protease009791 |
| Protein Name |
Signal peptidase complex catalytic subunit SEC11A EC 3.4.21.89 Endopeptidase SP18 Microsomal signal peptidase 18 kDa subunit SPase 18 kDa subunit SEC11 homolog A SEC11-like protein 1 SPC18 |
| Gene Name | SEC11A SEC11L1 SPC18 SPCS4A |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPAFHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAVDDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE |
| Enzyme Length | 179 |
| Uniprot Accession Number | P67812 |
| Absorption | |
| Active Site | ACT_SITE 56; /note=Charge relay system; /evidence=ECO:0000305|PubMed:34388369; ACT_SITE 96; /note=Charge relay system; /evidence=ECO:0000305|PubMed:34388369; ACT_SITE 122; /note=Charge relay system; /evidence=ECO:0000305|PubMed:34388369 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.; EC=3.4.21.89; Evidence={ECO:0000269|PubMed:34388369}; |
| DNA Binding | |
| EC Number | 3.4.21.89 |
| Enzyme Function | FUNCTION: Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (PubMed:34388369). Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids (PubMed:34388369). {ECO:0000269|PubMed:34388369}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (4); Chain (1); Mutagenesis (6); Region (1); Sequence conflict (5); Topological domain (2); Transmembrane (1) |
| Keywords | 3D-structure;Alternative splicing;Endoplasmic reticulum;Hydrolase;Membrane;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | Q08AM2; P06276 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P67811}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P67811}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | Electron microscopy (1) |
| Cross Reference PDB | 7P2P; |
| Mapped Pubmed ID | 17353931; 18396350; 19280057; 20711500; 21988832; 23995782; 26638075; 27859949; 2890159; 31163419; 32054332; 32564026; 3511473; |
| Motif | |
| Gene Encoded By | |
| Mass | 20,625 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |