| IED ID | IndEnz0002009793 |
| Enzyme Type ID | protease009793 |
| Protein Name |
Subtilisin-like protease SBT6.1 EC 3.4.21.- Site-1 protease AtS1P Subtilase subfamily 6 member 1 AtSBT6.1 |
| Gene Name | SBT6.1 S1P At5g19660 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKVLGEASSYPYRSCIIVVFLSVSLFWLRPSTYHPQQQNLNPENVTRLESENETKTNYIIRFKQYKPAKDHRIYLESKVRSGGWGWIERINPATKYPTDFGVLWIEESGKEAVVGEIERLEMVKDVNVEFKYQRVLLGGSFPDGKKRPGKIFTSMSFEEGTESSPMADTSNTTLNWSRHLLAQKTQVTSMFGADHLWKKGYTGAKVKMAIFDTGIRADHPHFRKIKERTNWTNEDTLNDNLGHGTFVAGVIAGRNPECLGFASDTEIYAFRVFTDAQVSYTSWFLDAFNYAIATDMDVLNLSIGGPDYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDNDDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSLSGTSVASPVVAGIVCLLVSVIPEARRKDLLNPASMKQALVEGAAKLSGPNMYEQGAGRVDLLESYEILKSYHPRASIFPSILDYNDCPYSWPFCRQPLYAGAMPIIFNTTILNGMGVIGYIESPPTWHPANEEGNLLSIHFKYPDVIWPWTGYLALHMQIKEEGAQFTGEIEGNVTVKVYSPPASGESGPRRSTCSLQLKLKVIPTPPRAKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHIMYNMLRDAGYYIETLGSPLTCFDAQQYGTLLMVDLEDDYFPEEIEKLRDDVINTGLGLVVFAEWYNVDTMVKMRFFDDNTRSWWTPVTGGANIPALNNLLASFGIAFGDKILNGDFSIDGEQSRYASGTNIVRFPAGGFLHTFPLLDSSESGATQNLLLTEASKEDPAVLGLLEIGEGRVGVYGDSNCLDSSHMVTNCYWLLKKMLDFSSSNIKDPVLFSKFAKRYSPVIIDEKQLPSRRTDVNFSTYSSVIGKELICESDSRFEVWGTKGYNLHVRGRNRRLPGYHGIDLGRGLNFTVESKRPTRWRSAKEGGELSSSRSKSLGGLFNRDEIDMPFLVPTRWIVLAGVVASGVLVLLSIWRIRQKRGRRRRASGSNRLA |
| Enzyme Length | 1038 |
| Uniprot Accession Number | Q0WUG6 |
| Absorption | |
| Active Site | ACT_SITE 212; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 243; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 409; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease that catalyzes the first step (site-1 cleavage) in the proteolytic activation of various factors, prior to site-2 cleavage. Part of a regulated intramembrane proteolysis (RIP) cascade. Cleaves BZIP17 and BZIP28 after the Arg-Arg-Ile-Leu (RRIL) motif. May cleave BZIP49 after the RRIL motif. Targets the membrane-associated BZIP17 factor, which functions as a stress sensor and transducer in a signaling pathway that resembles an ER stress response. Following salt stress, BZIP17 is cleaved by SBT6.1 (S1P) and S2P at the C-terminus and the N-terminal bZIP component is translocated to the nucleus, where it activates the expression of salt stress response genes (PubMed:17662035). Cleaves the pectinesterases PME1 after the Arg-Arg-Leu-Met (RRLM) and Arg-Arg-Leu-Leu (RRLL) motifs, and PME5 after the Arg-Arg-Leu-Leu (RRLL) and Arg-Lys-Leu-Met (RKLM) motifs. This processing and C-terminus release occurs in the Golgi apparatus and is required for cell wall targeting of pectinesterases. Thus, SBT6.1 mediates the regulated release of mature pectinesterases from the Golgi (PubMed:19144003). Cleaves the peptide growth factor RALF23 after the Arg-Arg-Ile-Leu (RRIL) motif. This processing is required for RALF23 function in the negative regulation of brassinolide (BL)-mediated signaling pathway (e.g. BL-induced hypocotyl elongation and branching limitation) (PubMed:19473327). {ECO:0000269|PubMed:17662035, ECO:0000269|PubMed:19144003, ECO:0000269|PubMed:19473327}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Glycosylation (10); Propeptide (1); Sequence conflict (1); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305|PubMed:17662035, ECO:0000305|PubMed:20876872}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..30; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14697270; 19704771; 22335396; 27315833; 28104890; 28407373; 30514814; |
| Motif | |
| Gene Encoded By | |
| Mass | 116,151 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |