| IED ID | IndEnz0002009824 |
| Enzyme Type ID | protease009824 |
| Protein Name |
Stringent starvation protein B Adapter protein SspB Specificity-enhancing factor SspB |
| Gene Name | sspB b3228 JW3197 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK |
| Enzyme Length | 165 |
| Uniprot Accession Number | P0AFZ3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth.; FUNCTION: Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (7); Chain (1); Compositional bias (1); Helix (4); Mutagenesis (4); Region (3); Turn (2) |
| Keywords | 3D-structure;Direct protein sequencing;Reference proteome;Stress response |
| Interact With | |
| Induction | INDUCTION: By amino acid starvation. {ECO:0000269|PubMed:8022275}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1OX8; 1OX9; 1YFN; 2DS8; |
| Mapped Pubmed ID | 15690043; 16606699; 24561554; |
| Motif | |
| Gene Encoded By | |
| Mass | 18,262 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |