| IED ID | IndEnz0002009839 |
| Enzyme Type ID | protease009839 |
| Protein Name |
V-type proton ATPase catalytic subunit A V-ATPase subunit A EC 7.1.2.2 V-ATPase 69 kDa subunit Vacuolar H + -ATPase subunit A Vacuolar proton pump subunit alpha |
| Gene Name | VHA-A At1g78900 F9K20.5 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MPAFYGGKLTTFEDDEKESEYGYVRKVSGPVVVADGMAGAAMYELVRVGHDNLIGEIIRLEGDSATIQVYEETAGLTVNDPVLRTHKPLSVELGPGILGNIFDGIQRPLKTIARISGDVYIPRGVSVPALDKDCLWEFQPNKFVEGDTITGGDLYATVFENTLMNHLVALPPDAMGKITYIAPAGQYSLKDTVIELEFQGIKKSYTMLQSWPVRTPRPVASKLAADTPLLTGQRVLDALFPSVLGGTCAIPGAFGCGKTVISQALSKYSNSDAVVYVGCGERGNEMAEVLMDFPQLTMTLPDGREESVMKRTTLVANTSNMPVAAREASIYTGITIAEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLAARLASFYERAGKVKCLGGPERNGSVTIVGAVSPPGGDFSDPVTSATLSIVQVFWGLDKKLAQRKHFPSVNWLISYSKYSTALESFYEKFDPDFINIRTKAREVLQREDDLNEIVQLVGKDALAEGDKITLETAKLLREDYLAQNAFTPYDKFCPFYKSVWMMRNIIHFYNLANQAVERAAGMDGQKITYTLIKHRLGDLFYRLVSQKFEDPAEGEDTLVEKFKKLYDDLNAGFRALEDETR |
| Enzyme Length | 623 |
| Uniprot Accession Number | O23654 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate; Xref=Rhea:RHEA:57720, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.1.2.2; |
| DNA Binding | |
| EC Number | 7.1.2.2 |
| Enzyme Function | FUNCTION: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 252..259; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Nucleotide binding (1) |
| Keywords | ATP-binding;Hydrogen ion transport;Ion transport;Membrane;Nucleotide-binding;Reference proteome;Translocase;Transport;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:17151019}; Peripheral membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12766230; 12938931; 14760709; 15028209; 15047896; 15215502; 15539469; 15610354; 15618420; 15971193; 16061227; 16199612; 16242667; 16247729; 16287169; 16461379; 16461582; 16581873; 16618929; 16635983; 16636467; 16679420; 17028149; 17317660; 17426018; 17432890; 17644812; 17710654; 17872919; 17875927; 17916636; 18324730; 18431481; 18775970; 20118269; 20133698; 20836890; 21166475; 21798377; 22943363; 23281391; 23615222; 23738980; 23782494; 24556609; 27247031; 28627464; 30261913; 32753431; |
| Motif | |
| Gene Encoded By | |
| Mass | 68,812 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:57720 |
| Cross Reference Brenda |