| IED ID | IndEnz0002009883 |
| Enzyme Type ID | protease009883 |
| Protein Name |
Puromycin-sensitive aminopeptidase PSA EC 3.4.11.14 Cytosol alanyl aminopeptidase AAP-S Meiotic prophase aminopeptidase 1 |
| Gene Name | MPA1 At1g63770 F24D7.4 T12P18.21 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDAPKEIFLKNYTKPDYYFETVDLSFSLGEEKTIVSSKIKVSPRVKGSSAALVLDGHDLKLLSVKVEGKLLKEGDYQLDSRHLTLPSLPAEESFVLEIDTEIYPHKNTSLEGLYKSSGNFCTQCEAEGFRKITFYQDRPDIMAKYTCRVEGDKTLYPVLLSNGNLISQGDIEGGRHYALWEDPFKKPCYLFALVAGQLVSRDDTFTTRSGRQVSLKIWTPAEDLPKTAHAMYSLKAAMKWDEDVFGLEYDLDLFNIVAVPDFNMGAMENKSLNIFNSKLVLASPETATDADYAAILGVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDMGSRTVKRIADVSKLRIYQFPQDAGPMAHPVRPHSYIKMDNFYTVTVYEKGAEVVRMYKTLLGTQGFRKGIDLYFERHDEQAVTCEDFFAAMRDANNADFANFLQWYSQAGTPVVKVVSSYNADARTFSLKFSQEIPPTPGQPTKEPTFIPVVVGLLDSSGKDITLSSVHHDGTVQTISGSSTILRVTKKEEEFVFSDIPERPVPSLFRGFSAPVRVETDLSNDDLFFLLAHDSDEFNRWEAGQVLARKLMLNLVSDFQQNKPLALNPKFVQGLGSVLSDSSLDKEFIAKAITLPGEGEIMDMMAVADPDAVHAVRKFVRKQLASELKEELLKIVENNRSTEAYVFDHSNMARRALKNTALAYLASLEDPAYMELALNEYKMATNLTDQFAALAALSQNPGKTRDDILADFYNKWQDDYLVVNKWFLLQSTSDIPGNVENVKKLLDHPAFDLRNPNKVYSLIGGFCGSPVNFHAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSLAA |
| Enzyme Length | 883 |
| Uniprot Accession Number | Q8H0S9 |
| Absorption | |
| Active Site | ACT_SITE 302; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by puromycin and DAMPAQ-22. {ECO:0000269|PubMed:15522847}. |
| Binding Site | BINDING 125; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides.; EC=3.4.11.14; |
| DNA Binding | |
| EC Number | 3.4.11.14 |
| Enzyme Function | FUNCTION: Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. Plays an essential role during prophase I of meiosis. Required for correct meiotic reconbination in both male and female gametophytes. {ECO:0000269|PubMed:15522847, ECO:0000269|PubMed:17151235}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (1); Chain (1); Erroneous gene model prediction (3); Metal binding (3); Region (1); Site (1) |
| Keywords | Alternative splicing;Aminopeptidase;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 27820795; |
| Motif | |
| Gene Encoded By | |
| Mass | 99,159 |
| Kinetics | |
| Metal Binding | METAL 301; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 305; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 324; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |