| IED ID | IndEnz0002009928 |
| Enzyme Type ID | protease009928 |
| Protein Name |
Serine protease inhibitor 88Ea Serpin 88Ea |
| Gene Name | Spn88Ea Spn5 CG18525 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MHILSISLMAVLPAIALAGLCGVEPDAGLLDQRLNLYKGQQNFAVSMLNVIRQSTPNENVFFSPYSTYHALLLAYFGSSGDTEKELAKVLHLDWADSKEVVRSAYILEKMNRKERQSKMPLEFSSADRIFFANDLHVTECARNRLAEEVQQIDFKSQTEESRKQINDWIAKQTHDQIRNMLSADEITPRTRLVLANAAYLKGQWLSQFKTEKTVPMPFYTSPSNYSLVSMMQQKGTFLLNVDEQLRAHVLQLPYRTVFESQEKEDSSPDENSDISMVLILPPFNSNSLEDVLSRLNADSLDDSLKQAMPREIEVSLPKFEFEQRLELNPILAKMGVSKMFDESVATFDDLTSETISIGDSKHVAKIKVDEEGSTAAAATVLFTYRSARPVEPAKFECNHPFLFVIYDRTSRSILFTGIYRDPKTIKQ |
| Enzyme Length | 427 |
| Uniprot Accession Number | Q9VFC2 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Serine protease inhibitor with activity toward trypsin (PubMed:18956323). Negatively regulates the Toll signaling pathway and suppresses the expression of the antifungal peptide drosomycin (PubMed:19581577). Its negative regulation of the Toll signaling pathway also results in the inhibition of the melanization immune response via the phenoloxidase (PPO1) cascade (PubMed:19581577). Essential for unfolding and expansion of the wings after emergence from the pupal case (PubMed:18956323). May regulate the Toll pathway by blocking the proteolysis of the Toll ligand spz (PubMed:19581577). {ECO:0000269|PubMed:18956323, ECO:0000269|PubMed:19581577}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (1); Sequence conflict (2); Signal peptide (1); Site (1) |
| Keywords | Glycoprotein;Immunity;Melanin biosynthesis;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19581577}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11606746; 11742098; 12032070; 12364793; 14605208; 14667416; 15056610; 15466469; 18077584; 19109990; 19343724; 19849829; 20220848; 20371351; 20980240; 21074052; 21480662; 21683256; 21940639; 23071443; 23840627; 23944235; 23972280; 25312911; 25687947; 26494844; 26526100; 27357258; 27894253; 28067623; 29265369; 29804808; 30957223; 31255773; 31520348; 31722958; 32677169; 33782689; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,476 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |