| IED ID | IndEnz0002009936 |
| Enzyme Type ID | protease009936 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 15 EC 3.4.19.12 Deubiquitinating enzyme 15 Protein SUPPRESSOR 2 OF DA1 Ubiquitin thioesterase 15 Ubiquitin-specific protease 15 AtUBP15 |
| Gene Name | UBP15 SOD2 At1g17110 F20D23.20 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MLEPRGADIPILFLVLVVLPVVAYILLGKWSNISEKRVRANLLAQMAAEEALRAETVVNADRGVRFESVATENRAQRTRTKTVSAGGGAVRAEFDAGARETVAEQRSDSVTATCGVTVVAPVNNNELHVCARCFGPAKTRCSRCKSVRYCSGKCQIIHWRVAHKDECVPVESCSSSSERVSFEKDSVLYDHGMDSTMYSNNTTQAAKGKTSKSSVDFASLGISQNDITPQINTQGRKSVGKQHSSKANRESCRRDSATVFDSSDEAASAGGDNKTSHIKHKSRGNSYAAETNPRRHSVDSSAVQMNGQSFVSGMQESHKHENNLGVRSSFGCPNTQYPSNGTRTATLPRTGINKSGEQSCTETSKKGQVAAVSKTVRSKDTGISEESNGISSTMGIMKMMGLRNSTKHDDRYKNLKMLFPYEEFLKFFQCEVFDLSPRGLVNCGNSCYANAVLQSLTCTKPLVAYLLRRSHSRSCSGKDWCLMCELEQHVMMLRESGGPLSASRILSHMRSINCQIGDGSQEDAHEFLRLLVASMQSICLERLGGETKVDPRLQETTLVQHMFGGRLRSKVKCLRCDHESERYENIMDLTLEIYGWVESLQDALTQFTRPEDLDGENMYRCSRCAGYVRARKELSIHEAPNILTIVLKRFQEGRYGKINKCISFPEMLDMIPFMTRTGDVPPLYMLYAVIVHLDTLNASFSGHYISYVKDLRGNWYRIDDSEIHPVPMTQVMSEGAYMLFYMRSYPRPQRGEHNGKAPVHHSQPRNEMKEQRKPVNRFKPRADHKNTESSSSEWSLFTSSDEASFTTESTRDSFSTIDYTDVCHVVDSSSPFAIFNNVYHNVEPSPHNTVACRMFSGTKPETRYFVEQETNHNNTVVLDATPSLYPIPAPYPPHDYYDQSMYVNYETNPEFNNGQDQDRTYSYW |
| Enzyme Length | 924 |
| Uniprot Accession Number | Q9FPS9 |
| Absorption | |
| Active Site | ACT_SITE 447; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10092; ACT_SITE 703; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10092 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:18485060}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (Probable). Involved in the regulation of organ size. Acts as positive regulator of cell proliferation. Possesses deubiquitinating enzyme activity in vitro. The enzyme activity of UBP15 is required for its function in regulation of cell proliferation (PubMed:18485060). Functions antagonistically in a common pathway with DA1 to regulate seed size. Acts maternally to regulate seed size by promoting cell proliferation in the integuments of ovules and developing seeds. Functions independently of DA2 and BB (PubMed:24585836). {ECO:0000269|PubMed:18485060, ECO:0000269|PubMed:24585836, ECO:0000305|PubMed:18485060}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (5); Domain (1); Erroneous gene model prediction (1); Metal binding (8); Mutagenesis (1); Region (3); Sequence conflict (6); Zinc finger (1) |
| Keywords | Alternative splicing;Cytoplasm;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18485060}. Nucleus {ECO:0000269|PubMed:18485060}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 103,677 |
| Kinetics | |
| Metal Binding | METAL 130; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 133; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 141; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 144; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 150; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 154; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 163; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 167; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134 |
| Rhea ID | |
| Cross Reference Brenda |